概述

  • 产品名称
  • 描述
    兔多克隆抗体to Stella
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 13 amino acid synthetic peptide from near the carboxy terminus of human Stella (NP_954980).

  • 阳性对照
    • 293 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab74531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

靶标

  • 功能
    May play a role in maintaining cell pluripotentiality.
  • 组织特异性
    Low expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
  • 发展阶段
    Expressed in fetal ovary.
  • 细胞定位
    Nucleus. Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 形式
    Stella is a gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage.
  • 别名
    • Compaction associated protein 1 antibody
    • Developmental pluripotency associated 3 antibody
    • Developmental pluripotency associated protein 3 antibody
    • Developmental pluripotency-associated protein 3 antibody
    • Dppa3 antibody
    • DPPA3_HUMAN antibody
    • PGC7 antibody
    • Stella related protein antibody
    • Stella-related protein antibody
    see all

图片

  • Lane 1 : Anti-Stella antibody (ab74531) at 1 µg/ml
    Lane 2 : Anti-Stella antibody (ab74531) at 2 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 18 kDa
    Observed band size : 18 kDa

实验方案

文献

This product has been referenced in:
  • Hu X  et al. Stem cells derived from human first-trimester umbilical cord have the potential to differentiate into oocyte-like cells in vitro. Int J Mol Med 35:1219-29 (2015). Read more (PubMed: 25760093) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Gonad)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9
Permeabilization
Yes - 0.05% triton X in PBS
Specification
Mouse Gonad
Fixative
Paraformaldehyde
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提交于 Nov 24 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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