概述

  • 产品名称Staurosporine
  • 描述
    Protein kinase inhibitor
  • 生物学描述Potent, cell-permeable, broad spectrum inhibitor of protein kinases. Kinases inhibited include: protein kinase C (IC50 = 3 nM), cAMP-dependent protein kinase (IC50 = 8 nM) and p60v-src (IC50 = 6 nM).
  • 纯度> 98%

性能

  • 化学名称[9S-(9α,10β,11β,13α)]-2,3,10,11,12,13-Hexahydro-10-methoxy-9-methyl-11-(methylamino)-9,13-epoxy-1H,9H-diindolo[1,2,3-gh:3,2,1-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one
  • 分子量466.54
  • 化学结构Chemical Structure
  • 分子式C28H26N4O3
  • CAS编号62996-74-1
  • PubChem识别号44259
  • 存放说明Store at +4°C. Store under Desiccating conditions. The product can be stored for up to 12 months.
  • 溶解度概述Soluble in DMSO to 50 mM
  • 处理

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILESO=C6NCc5c2c4n(c1ccccc12)[C@@]3(C)O[C@H](C[C@@H](NC)[C@H]3OC)n7c4c(c56)c8ccccc78
  • 来源

    Synthetic

  • 研究领域

    Staurosporine 图像

    • HeLa and Jurkat cells were treated with 1 µM Staurosporine (STS) (ab120056) for 4 hours in complete cell culture media to induce apoptosis and cleaved PARP protein. Untreated and STS treated HeLa and Jurkat lysates were prepared in 1X Cell Extraction Buffer PTR and tested the Cleaved PARP SimpleStepTM ELISA. Raw OD 450 nm values are shown for 500 µg/mL lysate loads.
    • Lane 1: HeLa, vehicle (DMSO) treated for 4 hours Lane 2: HeLa 1 µM staurpsorine (ab120056), 4 hours Load 20 µg/lane 5% milk/PBST for block and antibody diluent Primary antibodies (2 hours, room temp) All lanes: ab136812 250X Primary Antibodies Cocktail, 1/250 dilution Secondary antibodies (1 hour, room temp) All lanes: ab136812 100X HRP-Conjugated Secondary Antibodies Cocktail, 1/100 dilution

    • Example of HeLa staurosporine (ab120056) treated cell lysate titration. Background-subtracted data values (mean +/- SD) are graphed.
    • Example of IC50 determination. HeLa cells were treated with a dose titration of Staurosporine for 4 hours in complete media. Cells were cultured and treated in a 96-well cell culture microtiter plate. Lysates were prepared by direct in-well lysis without media removal: 2X Cell Extraction Buffer PTR was added to an equal volume of media and then resulting lysate was used directly in the Cleaved PARP SimpleStepTM ELISA assay. Raw values for triplicate measurements are plotted. The calculated IC50 is 0.77 µM.

    • Demonstration of Cleaved PARP capture antibody specificity by western blot assay. 20 µg of HeLa extracts that were untreated or treated for 4 hours with 1 µM Staurosporine were analyzed by western blot. The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, Staurosporine treatment is required to detect cleaved PARP protein, as observed in the SimpleStep ELISA.

    Staurosporine (ab120056)参考文献

    This product has been referenced in:
    • Gadbois DM  et al. Staurosporine is a potent inhibitor of p34cdc2 and p34cdc2-like kinases. Biochem Biophys Res Commun 184:80-5 (1992). Read more (PubMed: 1567459) »
    • Rüegg UT & Burgess GM Staurosporine, K-252 and UCN-01: potent but nonspecific inhibitors of protein kinases. Trends Pharmacol Sci 10:218-20 (1989). Read more (PubMed: 2672462) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your response.

    I would recommend preparing a 1 mM stock solution and using it at a 1 ul concentration in cell medium.



    Wherever possible, you should prepare and use solutions on the same day. However, if you ...

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    Just to confirm that our Lab has finished a quality control (QC) analysis of the compound, which it has passed. We are therefore confident that the compound remains of high purity.

    I can certainly offer you a replacement vial and would sugges...

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    Thank you for getting back to me with some further details.

    Our Lab is currently undertaking a quality control analysis on this batch of the compound to ensure that it remains of high purity. We expect a result back tomorrow although I expect...

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    Thank you for the clarification and for your quick response.

    It seems that the staurosporine is used at the right concentration for these cells so we are unsure why they are not detecting any apoptosis.

    Would you be so kind to confi...

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    Thank you for getting back to me and for providing some further details.

    I have discussed your enquiry with my colleagues in the Lab. Could you please send details of the calculations used to reconstitute the solution.

    We are a bit ...

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    Thank you for your enquiry and your interest in our products.

    We have sold several vials of this product and not received any complaints. Therefore we are not aware of any-quality-related issue regarding ab120056.

    It would be much a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"