概述

  • 产品名称Anti-Staufen抗体
    参阅全部 Staufen 一抗
  • 描述
    兔多克隆抗体to Staufen
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide: AKALRILQNE PLPERLEVNG RESEEENLNK SEISQVFEIA LKRNLPVNFE, corresponding to amino acids 73-122 of Human Staufen

  • 阳性对照
    • HepG2 cell lysate; human intestinal tissue.

性能

应用

Our Abpromise guarantee covers the use of ab50914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 63 kDa.Can be blocked with Human Staufen peptide (ab111678). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Binds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.
  • 组织特异性Widely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.
  • 序列相似性Contains 3 DRBM (double-stranded RNA-binding) domains.
  • 结构域One of the DRDB could be involved in RER binding.
    The C-terminal contains the tubulin binding domain (TBD).
  • 细胞定位Cytoplasm. Rough endoplasmic reticulum. Localizes exclusively with the rough reticulum endoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Double stranded RNA binding protein Staufen homolog 1 antibody
    • Double stranded RNA binding protein Staufen homolog antibody
    • Double-stranded RNA-binding protein Staufen homolog 1 antibody
    • FLJ25010 antibody
    • MGC124588 antibody
    • STAU antibody
    • STAU1 antibody
    • STAU1_HUMAN antibody
    • staufen antibody
    • Staufen RNA binding protein (Drosophila) antibody
    • Staufen RNA binding protein homolog 1 antibody
    • Staufen, RNA binding protein, homolog 1 (Drosophila) antibody
    see all

Anti-Staufen antibody 图像

  • Lane 1 : Marker
    Lane 2 : Anti-Staufen antibody (ab50914) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 63 kDa
    Gel concentration 12%
  • ICC/IF image of ab50914 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50914, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human intestine tissue labelling Staufen with ab50914 at 8µg/ml. Arrows indicate positive staining of epithelial cells of intestinal villi.

Anti-Staufen antibody (ab50914)参考文献

This product has been referenced in:
  • Vance C  et al. ALS mutant FUS disrupts nuclear localization and sequesters wild-type FUS within cytoplasmic stress granules. Hum Mol Genet 22:2676-88 (2013). Read more (PubMed: 23474818) »
  • Todd AG  et al. SMN, Gemin2 and Gemin3 associate with beta-actin mRNA in the cytoplasm of neuronal cells in vitro. J Mol Biol 401:681-9 (2010). Read more (PubMed: 20620147) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application CLIP
Sample Human Cell lysate - whole cell (HEK 293T)
Specification HEK 293T
Type iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control Protein A dynabeads without antibody bound
Immuno-precipitation step Other - Protein A dynabeads
Username

Abcam user community

Verified customer

提交于 Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (293T cell)
Loading amount 10 µg
Specification 293T cell
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Ms. Yeon ju Nam

Verified customer

提交于 May 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"