Anti-Stathmin 1 (phospho S16)抗体(ab47328)

概述

  • 产品名称Anti-Stathmin 1 (phospho S16)抗体
    参阅全部 Stathmin 1 一抗
  • 描述
    兔多克隆抗体to Stathmin 1 (phospho S16)
  • 特异性This antibody detects endogenous levels of Stathmin 1 only when phosphorylated at serine 16. (Human: Ser16; Mouse: Ser16; Rat: Ser16).
  • 经测试应用适用于: WB, ICC/IF, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Stathmin 1. synthesized phosphopeptide derived from human Stathmin 1 around the phosphorylation site of serine 16 (R-A-SP-G-Q)
    Database link: P16949

  • 阳性对照
    • human breast carcinoma

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab47328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000.
ICC/IF 1/100 - 1/500.
IHC-P 1/50 - 1/100.
ELISA 1/10000.

靶标

  • 功能Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
  • 组织特异性Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
  • 序列相似性Belongs to the stathmin family.
    Contains 1 SLD (stathmin-like) domain.
  • 翻译后修饰Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C1orf215 antibody
    • Lag antibody
    • LAP 18 antibody
    • LAP18 antibody
    • Leukemia associated phosphoprotein p18 antibody
    • Leukemia-associated phosphoprotein p18 antibody
    • Metablastin antibody
    • Oncoprotein 18 antibody
    • OP 18 antibody
    • Op18 antibody
    • p18 antibody
    • p19 antibody
    • Phosphoprotein 19 antibody
    • Phosphoprotein p19 antibody
    • pp17 antibody
    • pp19 antibody
    • PR22 antibody
    • Pr22 protein antibody
    • Prosolin antibody
    • Protein Pr22 antibody
    • SMN antibody
    • Stathmin antibody
    • Stathmin1 antibody
    • STMN 1 antibody
    • Stmn1 antibody
    • STMN1_HUMAN antibody
    see all

Anti-Stathmin 1 (phospho S16) antibody 图像

  • ab47328 staining human breast carcinoma tissue by IHC-P (left hand panel). The right hand panel shows staining in the presence of phospho-peptide.
  • ab47328 staining Stathmin1 (phospho S16) in Rat hippocampul neurons by Immunocytochemistry/ Immunofluorescence. Cultures of primary hippocampal neurons were prepared from embryonic day 18 rat embryos. The cells were transfected with GFP-shc (control shRNA vector) as control or GFP-sh2 Kidins220 / ARMS-specific shRNA, using serum-free medium with Lipofectamine. The cells were grown on coverslips and fixed for 5 min in 4% paraformaldehyde containing 4% sucrose in phosphate-buffered saline at 37 °C. Cells were then permeabilized with 0.2% Triton X-100 in phosphate-buffered saline during 5 min at room temperature. After blocking (5% bovine serum albumin in phosphate-buffered saline for 1 h), cells were incubated with the primary antibody. An Alexa Fluor®647 conjugated secondary antibody was used.

Anti-Stathmin 1 (phospho S16) antibody (ab47328)参考文献

This product has been referenced in:
  • Yip YY  et al. cAMP-dependent protein kinase and c-Jun N-terminal kinase mediate stathmin phosphorylation for the maintenance of interphase microtubules during osmotic stress. J Biol Chem 289:2157-69 (2014). Read more (PubMed: 24302736) »
  • Tian X  et al. Novel role of stathmin in microtubule-dependent control of endothelial permeability. FASEB J 26:3862-74 (2012). Read more (PubMed: 22700873) »

See all 3 Publications for this product

Product Wall

Thank you for your reply. I am sorry that the antibodies did not work for you as stated on the datasheet. I have processed your request to receive refunds for all 3 of these antibodies. Your credit note number is ********. If there is anything else I c...

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Thank you for contacting us. We have received your three western blot complaint questionnaires for ab47328, ab47398, and ab47399. These questionnaires refer to attachments with additional data, but unfortunately, we did not receive any attachments. ...

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Thank you for contacting us. The single amino acid difference is due to a nomenclature difference, in which the first methionine of a protein may or may not be included. Therefore, our antibodies detect the correct phosphorylation sites. I hope ...

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