概述

  • 产品名称Anti-Stathmin 1抗体
    参阅全部 Stathmin 1 一抗
  • 描述
    兔多克隆抗体to Stathmin 1
  • 特异性This antibody detects endogenous levels of total Stathmin 1 protein.
  • 经测试应用适用于: ELISA, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthesized non-phosphopeptide derived from human Stathmin 1 around the phosphorylation site of serine 37 (P-L-SP-P-P).

  • 阳性对照
    • Extracts from Jurkat cells and human breast carcinoma tissue

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab47472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
  • 组织特异性Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
  • 序列相似性Belongs to the stathmin family.
    Contains 1 SLD (stathmin-like) domain.
  • 翻译后修饰Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C1orf215 antibody
    • Lag antibody
    • LAP 18 antibody
    • LAP18 antibody
    • Leukemia associated phosphoprotein p18 antibody
    • Leukemia-associated phosphoprotein p18 antibody
    • Metablastin antibody
    • Oncoprotein 18 antibody
    • OP 18 antibody
    • Op18 antibody
    • p18 antibody
    • p19 antibody
    • Phosphoprotein 19 antibody
    • Phosphoprotein p19 antibody
    • pp17 antibody
    • pp19 antibody
    • PR22 antibody
    • Pr22 protein antibody
    • Prosolin antibody
    • Protein Pr22 antibody
    • SMN antibody
    • Stathmin antibody
    • Stathmin1 antibody
    • STMN 1 antibody
    • Stmn1 antibody
    • STMN1_HUMAN antibody
    see all

Anti-Stathmin 1 antibody 图像

  • All lanes : Anti-Stathmin 1 antibody (ab47472) at 1/500 dilution

    Lane 1 : Extract of Jurkat cells treated with PMA (1ng/ml, 15min)
    Lane 2 : Extract of Jurkat cells treated with PMA (1ng/ml, 15min) with synthetic peptide

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 17 kDa
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue in the presence (right) and absence (left) of synthetic peptide, using ab47472 at a 1/50 dilution.
  • ICC/IF image of ab47472 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47472, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Stathmin 1 antibody (ab47472)参考文献

This product has been referenced in:
  • Trott D  et al. Effect of phosphorylated hsp27 on proliferation of human endothelial and smooth muscle cells. Proteomics 9:3383-94 (2009). Read more (PubMed: 19562804) »

See 1 Publication for this product

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