Anti-STAT6 (phospho Y641)抗体(ab28829)

概述

  • 产品名称Anti-STAT6 (phospho Y641)抗体
    参阅全部 STAT6 一抗
  • 描述
    兔多克隆抗体to STAT6 (phospho Y641)
  • 特异性ab28829 recognises Phospho-STAT6(Tyr641).
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthesized phosphopeptide derived from human STAT6 around the phosphorylation site of Tyrosine 641.

  • 阳性对照
    • Breast carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab28829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 94 kDa.

靶标

  • 功能Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 12S1644 antibody
    • D12S1644 antibody
    • IL 4 STAT antibody
    • IL-4 Stat antibody
    • IL4 STAT antibody
    • Interleukin 4 Induced antibody
    • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
    • Signal transducer and activator of transcription 6 antibody
    • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
    • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
    • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
    • STAT 6 antibody
    • STAT interleukin4 induced antibody
    • STAT, interleukin4 induced antibody
    • Stat6 antibody
    • STAT6_HUMAN antibody
    • STAT6B antibody
    • STAT6C antibody
    • Transcription factor IL 4 STAT antibody
    see all

Anti-STAT6 (phospho Y641) antibody 图像



  • Predicted band size : 94 kDa

    Western blot analysis of lysates from HeLa cells treated with IL-4, using STAT6 (Phospho-Tyr641) Antibody. The lane on the right is blocked with the phospho peptide.

     

     

     

  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of breast carcinom using ab28829 preincubated with synthesised phospho-peptide.
  • ab28829 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.

    See Abreview

Anti-STAT6 (phospho Y641) antibody (ab28829)参考文献

ab28829 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Control and IL-13 Treated Mouse Lungs)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization No
Specification Control and IL-13 Treated Mouse Lungs
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10% NBF
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Ms. Premier Laboratory

Verified customer

提交于 Apr 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
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Abcam user community

Verified customer

提交于 Sep 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (Aorta)
Specification Aorta
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Human Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Sample Human Tissue sections (Aorta)
Specification Aorta
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization Yes - 0.1% Triton-X-100
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 08 2013

Thank you very much for having provided your feedback in the last survey on the resolution of the complaints. We completely understand your concern and apologize for the inconvenience caused by the faulty vial. This is obviously not what we expect neit...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

提交于 Apr 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"