概述

  • 产品名称Anti-STAT5b (phospho S731)抗体
    参阅全部 STAT5b 一抗
  • 描述
    兔多克隆抗体to STAT5b (phospho S731)
  • 经测试应用适用于: ICC/IF, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide based on human STAT5B around the phosphorylation site of serine 731 (A-P-SP-P-A).

  • 阳性对照
    • Human breast carcinoma tissue; extracts from RAW264.7 cells.
  • 常规说明


    ab52211 detects endogenous levels of STAT5B but only when phosphorylated at serine 731 (human) or serine 730 (mouse and rat).

性能

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应用

Our Abpromise guarantee covers the use of ab52211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.

靶标

  • 功能Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
  • 疾病相关Growth hormone insensitivity with immunodeficiency
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Signal transducer and activator of transcription 5B antibody
    • STA5B_HUMAN antibody
    • STAT5 antibody
    • Stat5b antibody
    • Transcription factor STAT5B antibody
    see all

Anti-STAT5b (phospho S731) antibody 图像

  • All lanes : Anti-STAT5b (phospho S731) antibody (ab52211) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7 cells treated with EGF (200ng/ml, 30min)
    Lane 2 : Extracts from RAW264.7 cells treated with EGF (200ng/ml, 30min), ab52211 was pre-incubated with immunising (blocking) peptide


    Predicted band size : 90 kDa
  • Paraffin-embedded human breast carcinoma tissue stained for STAT5b (phospho S731), using ab52211 (1/100). The right hand panel represents a negative control where ab52211 was pre-incubated with the immunising (blocking) peptide.
  • ICC/IF image of ab52211 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52211, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-STAT5b (phospho S731) antibody (ab52211)参考文献

This product has been referenced in:
  • Chen AY  et al. Productive Parvovirus B19 Infection of Primary Human Erythroid Progenitor Cells at Hypoxia Is Regulated by STAT5A and MEK Signaling but not HIFa. PLoS Pathog 7:e1002088 (2011). WB ; Human . Read more (PubMed: 21698228) »

See 1 Publication for this product

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