概述

  • 产品名称Anti-STAT5b抗体
    参阅全部 STAT5b 一抗
  • 描述
    兔多克隆抗体to STAT5b
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 179-228 (LRIQAQFGPLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVE L) of Human STAT5b (NP_036580).

  • 阳性对照
    • Transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab86273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
  • 疾病相关Growth hormone insensitivity with immunodeficiency
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Signal transducer and activator of transcription 5B antibody
    • STA5B_HUMAN antibody
    • STAT5 antibody
    • Stat5b antibody
    • Transcription factor STAT5B antibody
    see all

Anti-STAT5b antibody 图像

  • Anti-STAT5b antibody (ab86273) at 1 µg/ml (5% skim milk/ PBS buffer) + transfected 293T cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
  • ab86273 staining STAT5b in placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab86273 staining STAT5b in prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab86273 staining STAT5b in testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-STAT5b antibody (ab86273)参考文献

ab86273 has not yet been referenced specifically in any publications.

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