Anti-STAT5a (phospho Y694)抗体[6.F1] (ab106095)
Key features and details
- Mouse monoclonal [6.F1] to STAT5a (phospho Y694)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-STAT5a (phospho Y694)抗体[6.F1]
参阅全部 STAT5a 一抗 -
描述
小鼠单克隆抗体[6.F1] to STAT5a (phospho Y694) -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to Mouse STAT5a (phospho Y694).
Database link: P42230 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
NK92 cell lysate; Human breast tissue; 3T3 cells. -
克隆
单克隆 -
克隆编号
6.F1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106095于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 91 kDa.
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|
IHC-P | (1) |
Use a concentration of 20 - 40 µg/ml.
|
ICC/IF |
1/50 - 1/1000.
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说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 91 kDa. |
IHC-P
Use a concentration of 20 - 40 µg/ml. |
ICC/IF
1/50 - 1/1000. |
靶标
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功能
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 6776 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 24918 Rat
- Omim: 601511 Human
- SwissProt: P42229 Human
- SwissProt: P42230 Mouse
- SwissProt: Q62771 Rat
- Unigene: 437058 Human
see all -
别名
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
see all
图片
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All lanes : Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095) at 1/1000 dilution (overnight at 4°C)
Lane 1 : NK92 cell lysate, untreated
Lane 2 : NK92 cell lysate, treated with 1Ku of IL2 (30min)
Secondary
All lanes : Gt-a-Mouse IgG DyLight™800 conjugated at 1/20000 dilution
Predicted band size: 91 kDa -
ab106095 at 20µg/ml staining STAT5a in Formalin-fixed, Paraffin-embedded Human breast tissue by Immunohistochemistry.
The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. -
ab106095 staining STAT5a in 3T3 cells by Immunofluorescence (red). Green staining represents tubulin.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab106095 被引用在 2 文献中.
- Wang S et al. Canonical and noncanonical regulatory roles for JAK2 in the pathogenesis of rheumatoid arthritis-associated interstitial lung disease and idiopathic pulmonary fibrosis. FASEB J 36:e22336 (2022). PubMed: 35522243
- Datta P et al. Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells. Cytotechnology 67:223-35 (2015). PubMed: 24468831