Anti-STAT5a (phospho Y694)抗体[6.F1] (ab106095)

概述

  • 产品名称
    Anti-STAT5a (phospho Y694)抗体[6.F1]
    参阅全部 STAT5a 一抗
  • 描述
    小鼠单克隆抗体[6.F1] to STAT5a (phospho Y694)
  • 特异性
    ab106095 is specific for STAT5a protein phosphorylated at Y694.
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    A synthetic peptide corresponding to residues surrounding Y694 of Mouse STAT5a protein.

性能

应用

Our Abpromise guarantee covers the use of ab106095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000. Predicted molecular weight: 91 kDa.
IHC-P Use a concentration of 20 - 40 µg/ml.
ICC/IF 1/50 - 1/1000.

靶标

  • 功能
    Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • 序列相似性
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰
    Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • 细胞定位
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Mammary gland factor antibody
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • STA5A_HUMAN antibody
    • STAT 5 antibody
    • STAT 5A antibody
    • STAT5 antibody
    • STAT5A antibody
    see all

图片

  • All lanes : Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095) at 1/1000 dilution (overnight at 4°C)

    Lane 1 : NK92 cell lysate, untreated
    Lane 2 : NK92 cell lysate, treated with 1Ku of IL2 (30min)

    Secondary
    Gt-a-Mouse IgG DyLight™800 conjugated at 1/20000 dilution

    Predicted band size : 91 kDa
  • ab106095 at 20µg/ml staining STAT5a in Formalin-fixed, Paraffin-embedded Human breast tissue by Immunohistochemistry.
    The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
  • ab106095 staining STAT5a in 3T3 cells by Immunofluorescence (red). Green staining represents tubulin.

文献

ab106095 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6 - using a pressure cooker
Permeabilization
Yes - Triton100-X
Specification
Mammary gland
Blocking step
MOM Mouse IgG blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
Fixative
Formaldehyde
Username

Flavia Ghiraldini

Verified customer

提交于 Sep 27 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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