Anti-STAT5a (phospho S725)抗体(ab63404)

概述

  • 产品名称Anti-STAT5a (phospho S725)抗体
    参阅全部 STAT5a 一抗
  • 描述
    兔多克隆抗体to STAT5a (phospho S725)
  • 特异性ab63404 detects STAT5a only when phosphorylated at serine 725.
  • 经测试应用适用于: ELISA, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human STAT5a around the phosphorylation site of serine 725 (A-P-SP-P-V).

  • 阳性对照
    • Human breast carcinoma tissue and HepG2 cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab63404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.

靶标

  • 功能Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Mammary gland factor antibody
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • STA5A_HUMAN antibody
    • STAT 5 antibody
    • STAT 5A antibody
    • STAT5 antibody
    • STAT5A antibody
    see all

Anti-STAT5a (phospho S725) antibody 图像

  • ab63404 at 1/50 - 1/100 dilution staining STAT5a in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • ab63404 at 1/500 - 1/1000 dilution staining STAT5a in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-STAT5a (phospho S725) antibody (ab63404)参考文献

ab63404 has not yet been referenced specifically in any publications.

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