产品名称 Anti-STAT5a抗体 参阅全部 STAT5a 一抗
特异性 ab31377 detects endogenous levels of STAT5A protein around Tyrosine 694.
ELISA, WB, IHC-P more details
Synthesized non-phosphopeptide derived from human STAT5A around the phosphorylation site of Tyrosine 694.
HeLa cell lysate, Breast carcinoma tissue.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS[without Calcium and Magnesium ions], 150mM Sodium chloride, pH 7.4
Concentration information loading...
纯度 Immunogen affinity purified
Corresponding phospho antibody
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 91 kDa.
Use at an assay dependent concentration.
功能 Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
序列相似性 Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
翻译后修饰 Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
细胞定位 Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Information by UniProt
Mammary gland factor antibody
Signal transducer and activator of transcription 5A antibody
Anti-STAT5a antibody 图像
Immunohistochemistry (Paraffin-embedded sections) - STAT5a antibody (ab31377)
Western blot - STAT5a antibody (ab31377)
Lane 1 : Anti-STAT5a antibody (ab31377) at 1/500 dilution Lane 2 : Anti-STAT5a antibody (ab31377) at 1/500 dilution (preincubated with synthesized peptide) Lane 1 : HeLa cell extract. Lane 2 : HeLa cell extract. Predicted band size : 91 kDa Observed band size : 89 kDa ( why is the actual band size different from the predicted?) Additional bands at : 83 kDa. We are unsure as to the identity of these extra bands.
Anti-STAT5a antibody (ab31377)参考文献
has not yet been referenced specifically in any publications.
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