Anti-STAT5 (phospho Y694)抗体(ab98338)

概述

  • 产品名称Anti-STAT5 (phospho Y694)抗体
    参阅全部 STAT5 一抗
  • 描述
    兔多克隆抗体to STAT5 (phospho Y694)
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Cow, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 - 750 of Human STAT5, phosphorylated at Y694.

  • 阳性对照
    • This antibody gave a positive signal in A431 EGF-treated whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab98338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

Anti-STAT5 (phospho Y694) antibody 图像

  • All lanes : Anti-STAT5 (phospho Y694) antibody (ab98338) at 1 µg/ml

    Lane 1 : EGF-Stimulated A431 Whole Cell Lysate
    Lane 2 : EGF-Stimulated A431 Whole Cell Lysate with immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 91 kDa
    Observed band size : 91 kDa
    Additional bands at : 185 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab98338 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab98338 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-STAT5 (phospho Y694) antibody (ab98338)参考文献

ab98338 has not yet been referenced specifically in any publications.

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