概述

  • 产品名称Anti-STAT5 (phospho Y694)抗体
    参阅全部 STAT5 一抗
  • 描述
    兔多克隆抗体to STAT5 (phospho Y694)
  • 特异性This antibody recognizes STAT5 (pY694) with phosphorylated sites of Tyr694. It does not cross react with non phosphospecific peptide.
  • 经测试应用适用于: ELISA, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow, Dog
  • 免疫原

    A synthetic peptide surrounding the epitope -DGYVK- with phosphorylation sites at Tyr694 of STAT5 protein from Human, Mouse and Rat origins.

  • 阳性对照
    • GH stimulated Mouse liver lysate and Human breast carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab97734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.01 - 0.1 µg/ml.
IHC-P 1/100. Staining of formalin fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
IP Use a concentration of 2 - 5 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 90 kDa.

靶标

Anti-STAT5 (phospho Y694) antibody 图像

  • Human breast carcinoma tissue stained with ab97734 at 1/100 for 10 minutes at room temperature.
  • All lanes : Anti-STAT5 (phospho Y694) antibody (ab97734) at 1/500 dilution

    Lane 1 : GH stimulated Mouse liver lysate
    Lane 2 : Negtive control


    Predicted band size : 90 kDa

Anti-STAT5 (phospho Y694) antibody (ab97734)参考文献

This product has been referenced in:
  • Tsuruyama T  et al. STAT5A Modulates Chemokine Receptor CCR6 Expression and Enhances Pre-B Cell Growth in a CCL20-Dependent Manner. J Cell Biochem 117:2630-42 (2016). Read more (PubMed: 27018255) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"