Anti-STAT5 (phospho Y694)抗体(ab13593)


  • 产品名称Anti-STAT5 (phospho Y694)抗体
    参阅全部 STAT5 一抗
  • 描述
    兔多克隆抗体to STAT5 (phospho Y694)
  • 特异性ab13593 recognises phosphorylated STAT5.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    This antibody was raised against a chemically synthesized phosphopeptide derived from the region of human STAT5a that contains tyrosine 694. The sequence is conserved in mouse and rat.

  • 阳性对照
    • 3T3 L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).



Our Abpromise guarantee covers the use of ab13593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 90.6 kDa).


  • 功能Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGF antibody
    • Signal transducer and activator of transcription 5A antibody
    • Signal Transducer and Activator of Transcription 5B antibody
    • STA5A_HUMAN antibody
    • STAT 5A antibody
    • STAT 5B antibody
    • STAT5 antibody
    • STAT5A antibody
    • STAT5B antibody
    • Transcription factor STAT5A antibody
    • Transcription factor STAT5B antibody
    see all

Anti-STAT5 (phospho Y694) antibody 图像

  • Predicted band size : 90.6 kDa

    Lysates prepared from 3T3-L1 cells left untreated (1) or treated with LIF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ab13593 for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, an appropriate secondary antibody was applied and the membrane was developed. The data show that only the peptide corresponding to STAT5 [pY694] blocks the signal, verifying the specificity of the antibody.

Anti-STAT5 (phospho Y694) antibody (ab13593)参考文献

This product has been referenced in:
  • Ahmed KB  et al. In vitro and in vivo characterization of SGI-1252, a small molecule inhibitor of JAK2. Exp Hematol 39:14-25 (2011). Read more (PubMed: 20934482) »
  • Tomita T  et al. Oncostatin M regulates secretoglobin 3A1 and 3A2 expression in a bidirectional manner. Am J Respir Cell Mol Biol 40:620-30 (2009). WB ; Mouse . Read more (PubMed: 18978304) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. The immunogen used for the phospho specific antibody is a synthetic peptide of around 10 amino acid residues long with the phospho site in the central area. The exact immunogenic sequence for this anitbody is consider...

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I have just received the following information from the source of ab13593 to answer your query: At the region surrounding tyrosine 694 is highly conserved. We actually tested mouse cell lysates and not human ones with this antibody, so we know that...

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