STAT4 (Tyr693) In-Cell ELISA试剂盒(ab126428)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Colorimetric
- Sample type: Adherent cells
- Reacts with: Mouse, Human
概述
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产品名称
STAT4 (Tyr693) In-Cell ELISA试剂盒
参阅全部 STAT4 试剂盒 -
检测方法
Colorimetric -
样品类型
Adherent cells -
检测类型
Cell-based (qualitative) -
检测时间
5h 10m -
实验步骤
Multiple steps standard assay -
种属反应性
与反应: Mouse, Human -
产品概述
ab126428 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of STAT4 (Tyr693) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human and mouse cell lines. By determining STAT4 protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.
In the STAT4 (Tyr693) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti Phospho-STAT4 (Tyr693) or Anti-STAT4 (primary antibody) is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-Mouse IgG or HRP-conjugated anti-rabbit IgG (secondary antibody) is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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平台
Microplate
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 x 96 tests Blocking Buffer Concentrate (5X) 1 x 20ml Fixing Solution 1 x 30ml HRP-conjugated Anti-Mouse IgG Concentrate 1 x 10µl Anti-Rabbit IgG Concentrate (Item I-1) 1 x 10µl Uncoated 96-well Microplate 1 unit Mouse Anti-STAT4 Concentrate (Item H) 1 x 7µl Quenching Buffer Concentrate (30x) 1 x 2ml Rabbit Anti-Phospho-STAT4 (Tyr693) Concentrate (Item G) 1 x 10µl Stop Solution 1 x 14ml TMB One-Step Substrate Reagent 1 x 12ml Wash Buffer A Concentrate (20X) 1 x 30ml Wash Buffer B Concentrate (20X) 1 x 30ml -
研究领域
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功能
Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling. -
疾病相关
Systemic lupus erythematosus 11
Rheumatoid arthritis -
序列相似性
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
翻译后修饰
Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity. -
细胞定位
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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别名
- Signal transducer and activator of transcription 4
- SLEB11
- STAT4
- STAT4_HUMAN
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数据库链接
- Entrez Gene: 6775 Human
- Entrez Gene: 20849 Mouse
- Omim: 600558 Human
- SwissProt: Q14765 Human
- SwissProt: P42228 Mouse
- Unigene: 735572 Human
- Unigene: 80642 Human
- Unigene: 1550 Mouse
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab126428 尚未被引用在任何文献中。