RabMAb

Anti-STAT4抗体[EP1900Y] (ab68156)

概述

  • 产品名称Anti-STAT4抗体[EP1900Y]
    参阅全部 STAT4 一抗
  • 描述
    兔单克隆抗体[EP1900Y] to STAT4
  • 经测试应用适用于: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide within Human STAT4 aa 700 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14765

  • 阳性对照
    • WB: Daudi, K562 and Hela cell lysates. IHC-P: Human tonsil tissue sections.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab68156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/5000. Detects a band of approximately 30, 86 kDa (predicted molecular weight: 86 kDa).
IP 1/20.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
  • 疾病相关Systemic lupus erythematosus 11
    Rheumatoid arthritis
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Signal transducer and activator of transcription 4 antibody
    • SLEB11 antibody
    • STAT4 antibody
    • STAT4_HUMAN antibody

Anti-STAT4 antibody [EP1900Y] 图像

  • All lanes : Anti-STAT4 antibody [EP1900Y] (ab68156) at 1/1000 dilution

    Lane 1 : Daudi cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab68156, at 1/100 dilution, staining STAT4 in paraffin embedded human tonsil tissue sections by Immunohistochemistry

Anti-STAT4 antibody [EP1900Y] (ab68156)参考文献

ab68156 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (human Vgamma9 Vdelta2 T cells)
Total protein in input 3e+006 cells
Immuno-precipitation step Other - Dynabeads (Protein G)
Specification human Vgamma9 Vdelta2 T cells
Username

Abcam user community

Verified customer

提交于 Nov 07 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Purified human Vgamma9 Vdelta2 T-cells)
Permeabilization Yes - Foxp3 Staining Buffer Set
Gating Strategy CD3 and V delta 2 positive population
Specification Purified human Vgamma9 Vdelta2 T-cells
Fixation Foxp3 Staining Buffer Set
Username

Abcam user community

Verified customer

提交于 Sep 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"