Anti-STAT4抗体(ab57822)

概述

  • 产品名称Anti-STAT4抗体
    参阅全部 STAT4 一抗
  • 描述
    小鼠单克隆抗体to STAT4
  • 经测试应用适用于: WB, ICC/IF, IHC-P, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-749 of Human STAT4

性能

应用

Our Abpromise guarantee covers the use of ab57822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 86 kDa.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.
  • 疾病相关Systemic lupus erythematosus 11
    Rheumatoid arthritis
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Signal transducer and activator of transcription 4 antibody
    • SLEB11 antibody
    • STAT4 antibody
    • STAT4_HUMAN antibody

Anti-STAT4 antibody 图像

  • STAT4 antibody (ab57822) used in immunofluorescence at 1-5ug/ml on HeLa cells.
  • STAT4 antibody (ab57822) used in immunohistochemistry at 2ug/ml on formalin fixed and paraffin embedded human ovary, clear cell carcinoma tissue.


  • Predicted band size : 86 kDa
    STAT4 antibody (ab57822) at 1ug/lane + A-549 cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab57822 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57822, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-STAT4 antibody (ab57822)参考文献

ab57822 has not yet been referenced specifically in any publications.

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