Anti-STAT3 (phospho S727)抗体(ab30647)

Abreviews (2)Q&A (1)Specific References (12)
Customer reviews and FAQs

概述

  • 产品名称Anti-STAT3 (phospho S727)抗体
    参阅全部 STAT3 一抗
  • 描述
    兔多克隆抗体to STAT3 (phospho S727)
  • 特异性ab30647 detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
  • 经测试应用适用于: IHC-FoFr, IHC-P, WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to Human STAT3 (phospho S727). The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
    Database link: P40763
    (Peptide available as ab43618, ab82053)

  • 阳性对照
    • IHC: breast carcinoma WB: HeLa cells lysate

性能

应用

Our Abpromise guarantee covers the use of ab30647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20950279
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).Can be blocked with Recombinant Human STAT3 protein (ab43618).
ELISA 1/10000.
IP Use at an assay dependent concentration.

靶标

  • 功能Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
  • 组织特异性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 疾病相关Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
    Autoimmune disease, multisystem, infantile-onset
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • ADMIO antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 (acute phase response factor) antibody
    • Signal transducer and activator of transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Anti-STAT3 (phospho S727) antibody 图像

  • Western blot - Anti-STAT3 (phospho S727) antibody (ab30647)
    Western blot - Anti-STAT3 (phospho S727) antibody (ab30647)
    All lanes : Anti-STAT3 (phospho S727) antibody (ab30647)

    Lane 1 : HeLa cells extract
    Lane 2 : HeLa cells extract with Synthesized non-phosphopeptide
    Lane 3 : HeLa cells extract with Synthesized phosphopeptide


    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody (ab30647)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT3 (phospho S727) antibody (ab30647)

    ab30647 at 1/50 dilution staining STAT3 (phospho S727) in human breast carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). The left image show staining by ab30647 without specific peptide while right image show staining after blocking with peptide.

Anti-STAT3 (phospho S727) antibody (ab30647)参考文献

This product has been referenced in:
  • Cui L  et al. Downregulation of B7-H4 in the MHCC97-H hepatocellular carcinoma cell line by arsenic trioxide. Mol Med Rep 13:2032-8 (2016). WB ; Human . Read more (PubMed: 26781180) »
  • Zhang L  et al. STAT3 is a key pathway in primary intraosseous squamous cell carcinoma arising from an odontogenic keratocyst. Am J Transl Res 7:1860-9 (2015). IHC-P ; Human . Read more (PubMed: 26692930) »

See all 12 Publications for this product

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Western blot abreview for Anti-STAT3 (phospho S727) antibody

 Good
Abreviews
Application Western blot
Loading amount 45 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Lung)
Specification Lung
Treatment Basel conditions
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
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Abcam user community

Verified customer

提交于 Oct 22 2013

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-STAT3 (phospho S727) antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Dr. Ruma Raha-Chowdhury

Verified customer

提交于 May 11 2012

Thank you for your reply.



I’m going to use a coctail which contains the following antibodies: AB30647, AB25533 and AB25024. So I’m looking for secondary antibody to AB30647, which will be in one tube with AB25024 and ...

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Thank you for your inquiry.

I strongly suggest to use a pre-absorbed secondary antibody so that the cross-binding to the two rat monoclonals can be minimized.

Since you are already using fluorescence in the green and red spectrum,...

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