Anti-STAT3 (phospho S727)抗体(ab30647)

概述

• 产品名称
  Anti-STAT3 (phospho S727)抗体
  参阅全部 STAT3 一抗
• 描述
  兔多克隆抗体to STAT3 (phospho S727)
• 特异性
  ab30647 detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
• 经测试应用
  适用于: IHC-FoFr, IHC-P, WB, ELISA, IPmore details
• 种属反应性
  与反应: Mouse, Rat, Dog, Human, African green monkey
  
• 免疫原

  Synthetic peptide corresponding to Human STAT3 (phospho S727). The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
  Database link: P40763
  (Peptide available as ab43618, ab82053)

• 阳性对照
  • IHC: Human breast carcinoma tissue. WB: HeLa and COS7 cell lysate.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
• 存储溶液
  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
  
  PBS without Mg2+ and Ca2+
• Concentration information loading...
• 纯度
  Protein A purified
• 纯化说明
  ab30647 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • STATs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Stem Cells
  • Embryonic Stem Cells
  • Intracellular

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • STATs

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • STAT family

  • Cardiovascular
  • Heart
  • Cardiogenesis
  • Transcription factors/regulators

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Transcription factors

  • Developmental Biology
  • Embryogenesis
  • Embryonic stem cells
  • Surface molecules

相关产品

• Assay kits
  • STAT3 Transcription Factor Assay Kit (Colorimetric) (ab207229)
• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Immunizing Peptide (Blocking)
  • Recombinant Human STAT3 protein (ab43618)
• Immunohistochemistry kits
  • EXPOSE Rabbit specific HRP/DAB detection IHC kit (ab80437)

应用

靶标

• 功能
  Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
• 组织特异性
  Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
• 疾病相关
  Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
  Autoimmune disease, multisystem, infantile-onset
• 序列相似性
  Belongs to the transcription factor STAT family.
  Contains 1 SH2 domain.
• 翻译后修饰
  Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
• 细胞定位
  Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.

• Target information above from: UniProt accession P40763 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 6774 Human
  • Entrez Gene: 20848 Mouse
  • Entrez Gene: 25125 Rat
  • Omim: 102582 Human
  • SwissProt: P40763 Human
  • SwissProt: P42227 Mouse
  • SwissProt: P52631 Rat
  • Unigene: 463059 Human

  • Unigene: 249934 Mouse
  • Unigene: 473190 Mouse
  • Unigene: 10247 Rat

  see all
• 别名
  • 1110034C02Rik antibody
  • Acute Phase Response Factor antibody
  • Acute-phase response factor antibody

  • ADMIO antibody
  • APRF antibody
  • AW109958 antibody
  • DNA binding protein APRF antibody
  • FLJ20882 antibody
  • HIES antibody
  • MGC16063 antibody
  • Signal transducer and activator of transcription 3 (acute phase response factor) antibody
  • Signal transducer and activator of transcription 3 antibody
  • STAT 3 antibody
  • Stat3 antibody
  • STAT3_HUMAN antibody

  see all

实验方案

• Immunoprecipitation protocols
• Immunohistochemistry protocols
• Western blot protocols