Anti-STAT3抗体[9D8] (ab119352)

概述

  • 产品名称Anti-STAT3抗体[9D8]
    参阅全部 STAT3 一抗
  • 描述
    小鼠单克隆抗体[9D8] to STAT3
  • 特异性Detects STAT3 from Human, murine, monkey and rat samples.
  • 经测试应用适用于: Flow Cyt, WB, IP, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Non Human Primates
  • 免疫原

    Recombinant fragment, corresponding to amino acids 665-770 of Human STAT3.

  • 阳性对照
    • HepG2, 293T, Jurkat, A431, U2OS, MCF7, A549, K562, NIH3T3, C2C12, and NRK whole cell lysates; U2OS lysate from STAT3 SMART pool siRNA transfected; HeLa cells; Human pancreas, brain tumour, colon cancer and stomach cancer tissues Flow Cyt: HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab119352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 0.1µg for 106 cells.
WB 1/5000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
IP Use at 2 µg/mg of lysate.
IHC-P 1/1600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100.

靶标

  • 功能Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity.
  • 组织特异性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 疾病相关Hyperimmunoglobulin E recurrent infection syndrome, autosomal dominant
    Autoimmune disease, multisystem, infantile-onset
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • ADMIO antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 (acute phase response factor) antibody
    • Signal transducer and activator of transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Anti-STAT3 antibody [9D8] 图像

  • Immunofluorescent analysis of STAT3 using ab119352 at 1/100 dilution (shown in green) in HeLa cells (formalin fixed cells permeabilized with 0.1% Triton X-100) using a DyLight 488 goat-anti-mouse secondary antibody (ab96879) at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.

  • Overlay histogram showing HeLa cells stained with ab119352 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween 20 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119352, 0.1μg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (ab37355, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/5000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : A431 cell lysate
    Lane 5 : U2OS cell lysate
    Lane 6 : MCF7 cell lysate
    Lane 7 : A549 cell lysate
    Lane 8 : K562 cell lysate
    Lane 9 : COS7 cell lysate
    Lane 10 : NIH3T3 cell lysate
    Lane 11 : C2C12 cell lysate
    Lane 12 : NRK cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    goat anti-mouse-HRP at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 88 kDa
    4-20% Tris-HCl polyacrylamide gel
  • Anti-STAT3 antibody [9D8] (ab119352) at 1/1000 dilution + HepG2 total lysate at 25 µg

    Secondary
    goat anti-mouse-HRP at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 88 kDa
    4-20% Tris-HCl polyacrylamide gel
  • All lanes : Anti-STAT3 antibody [9D8] (ab119352) at 1/1000 dilution

    Lane 1 : U2OS lysate from STAT3 SMART pool siRNA transfected
    Lane 2 : U2OS lysate from non-targeting control

    Lysates/proteins at 25 µg per lane.

    Secondary
    goat anti-mouse-HRP at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 88 kDa
    4-20% Tris-HCl polyacrylamide gel
  • Immunoprecipitation of STAT3 was performed on HepG2 cells. The antigen:antibody complex was formed by incubating 750µg whole cell lysate with 2µg of ab119352 overnight at 4°C. The immune-complex was captured, washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membrane and blocked with 5% Milk/TBS-0.1% Tween for at least 1 hour. Membranes were then probed with ab119352 at a dilution of 1/5000 overnight at 4°C. Membranes were washed in TBST and probed with IP detection reagent at a dilution of 1/2000 for at least one hour. Membranes were washed and chemiluminescent detection was performed.
  • ab119352, at 1/1600 dilution, staining STAT3 in Human pancreas tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.

  • ab119352, at 1/1600 dilution, staining STAT3 in Human brain tumor tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.

  • ab119352, at 1/1600 dilution, staining STAT3 in Human colon cancer tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.

  • ab119352, at 1/1600 dilution, staining STAT3 in Human stomach cancer tissue by Immunohistochemistry. Detection was performed using a goat anti-mouse HRP secondary antibody followed by colorimetric detection using DAB substrate. Tissues were counterstained with hematoxylin.

Anti-STAT3 antibody [9D8] (ab119352)参考文献

This product has been referenced in:
  • Zhao X  et al. Effects of 17-AAG on the cell cycle and apoptosis of H446 cells and the associated mechanisms. Mol Med Rep 14:1067-74 (2016). WB ; Human . Read more (PubMed: 27279418) »
  • Ma L  et al. Trap Effect of Three-Dimensional Fibers Network for High Efficient Cancer-Cell Capture. Adv Healthc Mater 4:838-43 (2015). Read more (PubMed: 25645204) »

See all 7 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Lung)
Gel Running Conditions Reduced Denaturing (4-12%)
Loading amount 22 µg
Specification Lung
Blocking step Odyssey TBS Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
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Pete Serbedzija

Verified customer

提交于 Jul 28 2016

Application Immunohistochemistry (Frozen sections)
Blocking step 0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification mouse embryonic spinal cord E11
Permeabilization Yes - 0.25%TritonX100
Fixative Paraformaldehyde
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Abcam user community

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提交于 Jul 28 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-15% TRIS-Glycine SDS PAGE)
Sample Mouse Tissue lysate - whole (1) mouse embryonic spinal cord E11, 2) HEK293H)
Specification 1) mouse embryonic spinal cord E11, 2) HEK293H
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 28 2014

The strain of mouse used for production of ab119352 was balb/c.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"