概述

  • 产品名称Anti-STAT2抗体
    参阅全部 STAT2 一抗
  • 描述
    兔多克隆抗体to STAT2
  • 特异性ab53149 detects endogenous levels of total STAT2 protein.
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthesized non phosphopeptide derived from human STAT2 around the phosphorylation site of tyrosine 690 (R-K-Y-L-K).

  • 阳性对照
    • WB: Extracts from HeLa cells IHC-P: Human lung carcinoma tissue

性能

应用

Our Abpromise guarantee covers the use of ab53149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 功能Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Tyrosine phosphorylated in response to IFN-alpha.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Homo sapiens interferon alpha induced transcriptional activator antibody
    • interferon alpha induced transcriptional activator antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • MGC59816 antibody
    • P113 antibody
    • signal transducer and activator of transcription 2 113kD antibody
    • Signal transducer and activator of transcription 2 antibody
    • STAT113 antibody
    • Stat2 antibody
    • STAT2_HUMAN antibody
    see all

Anti-STAT2 antibody 图像

  • Immunohistochemical analysis of STAT2 in paraffin embedded human lung carcinoma tissue using ab53149 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • All lanes : Anti-STAT2 antibody (ab53149) at 1/300 dilution

    Lane 1 : Extracts from HeLa cell treated with IFN (2500U/ml, 30 min) with no peptide
    Lane 2 : Extracts from HeLa cell treated with IFN (2500U/ml, 30 min) with peptide


    Predicted band size : 98 kDa

Anti-STAT2 antibody (ab53149)参考文献

This product has been referenced in:
  • Hill T  et al. IFNgamma mediates DUOX2 expression via a STAT-independent signaling pathway. Biochem Biophys Res Commun 395:270-4 (2010). WB ; Human . Read more (PubMed: 20381453) »

See 1 Publication for this product

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Thank you for your reply and for sending the protocol information. My colleague is out of the office today but I'll be happy to help you with this issue.

To improve the signal, we would recommend using 3% BSA for blocking and up to 1:300 dilu...

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Thank you for you reply. I am sorry to hear that the replacement antibody I had sent is also not giving any signal on WB. Can you please provide a few additional details of your protocol?

1) Is the over-expressed protein human STAT2 or is it ...

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