The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 98 kDa.
Use a concentration of 2.5 µg/ml.
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Belongs to the transcription factor STAT family. Contains 1 SH2 domain.
Tyrosine phosphorylated in response to IFN-alpha.
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
ab106094 at 2.5µg/ml staining STAT2 in Formalin fixed, Paraffin-embedded Human Skin (squamous epithelium) by Immunohistochemistry. Note: moderate to strong nuclear and faint to moderate cytoplasmic staining.
The image shows the localization of the antibody as the
precipitated red signal, with a hematoxylin purple nuclear