Anti-STAT1 (phospho Y701)抗体(ab30645)

概述

  • 产品名称Anti-STAT1 (phospho Y701)抗体
    参阅全部 STAT1 一抗
  • 描述
    兔多克隆抗体to STAT1 (phospho Y701)
  • 特异性ab30645 detects endogenous levels of STAT1 only when phosphorylated at Tyrosine 701.
  • 经测试应用适用于: ICC, ELISA, IP, IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human STAT1 around the phosphorylation site of Tyrosine 701.

  • 阳性对照
    • IHC: breast carcinoma WB: MC7 cells treated with EGF

性能

应用

Our Abpromise guarantee covers the use of ab30645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration.
ELISA 1/10000.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 87 kDa).
ICC/IF Use a concentration of 1 - 5 µg/ml.

靶标

  • 功能Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
  • 疾病相关Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
    Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
    Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
    ISGylated.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Signal transducer and activator of transcription 1 91kD antibody
    • CANDF7 antibody
    • DKFZp686B04100 antibody
    • IMD31A antibody
    • IMD31B antibody
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    • ISGF 3 antibody
    • ISGF-3 antibody
    • OTTHUMP00000163552 antibody
    • OTTHUMP00000165046 antibody
    • OTTHUMP00000165047 antibody
    • OTTHUMP00000205845 antibody
    • Signal transducer and activator of transcription 1 91kDa antibody
    • Signal transducer and activator of transcription 1 antibody
    • Signal transducer and activator of transcription 1, 91kD antibody
    • Signal transducer and activator of transcription 1-alpha/beta antibody
    • STAT 1 antibody
    • Stat1 antibody
    • STAT1_HUMAN antibody
    • STAT91 antibody
    • Transcription factor ISGF 3 components p91 p84 antibody
    • Transcription factor ISGF-3 components p91/p84 antibody
    see all

Anti-STAT1 (phospho Y701) antibody 图像

  • All lanes : Anti-STAT1 (phospho Y701) antibody (ab30645)

    Lane 1 : Untreated MCF7 lysate (5-30ug).
    Lane 2 : EGF treated MCF7 lysate (5-30ug).


    Predicted band size : 87 kDa
    Observed band size : 87 kDa
    Additional bands at : 47 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab30645 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30645, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab30645 staining STAT1 (phospho Y701) in murine intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 23°C; antigen retrieval was by heat mediation with a citrate buffer. Samples were incubated with primary antibody (1/400 in Tris Buffer Saline) for 16 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

Anti-STAT1 (phospho Y701) antibody (ab30645)参考文献

This product has been referenced in:
  • Wu Q  et al. Crosstalk of JNK1-STAT3 is critical for RAW264.7 cell survival. Cell Signal 26:2951-60 (2014). Mouse . Read more (PubMed: 25269780) »
  • Williams P  et al. A fusion of GMCSF and IL-21 initiates hypersignaling through the IL-21Ralpha chain with immune activating and tumoricidal effects in vivo. Mol Ther 18:1293-301 (2010). WB ; Mouse . Read more (PubMed: 20389285) »

See all 4 Publications for this product

Product Wall

Thank you fory our message which has been forwarded to me as my colleague ##### is away from the office today.

I am sorry I am not able to trace any previous correspondence from the email address you have used, and so I hope I can provide ad...

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Application Western blot
Sample Mouse Cell lysate - whole cell (Intestines)
Loading amount 15 µg
Specification Intestines
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Oct 04 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Aug 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Intestine)
Specification Intestine
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 May 30 2012

Thank you for your patience.


The antibody ab31369 detects both alpha and beta forms of STAT1 when it is not phosphorylated at Y701. We believe that your treatment is increasing the expression of total STAT1 as well as the phosphorylated ...

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Thank you for your emails and for your patience while I've looked into these questions.

I've heard back from the lab regarding the specificity of ab31369. The antibody should detect both the alpha and beta forms, thoughwe haven't specifically...

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This antibody has given good staining in IHC on paraffin embedded slides when heat induced antigen retrieval with citrate buffer pH6.0 has been used. Enzyme is not needed. Please find here below also the protocol details used for testing this antibod...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (Ovarian, Breast)
Specification Ovarian, Breast
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 02 2010

Application Western blot
Sample Human Cell lysate - whole cell (Human B-cell lymphomas)
Loading amount 20 µg
Specification Human B-cell lymphomas
Gel Running Conditions Reduced Denaturing (8% Acrylamide gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Prof. Paul Townsend

Verified customer

提交于 Mar 31 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"