RabMAb

Anti-STAT1 (phospho S727)抗体[EPR3146] (ab109461)

概述

  • 产品名称Anti-STAT1 (phospho S727)抗体[EPR3146]
    参阅全部 STAT1 一抗
  • 描述
    兔单克隆抗体[EPR3146] to STAT1 (phospho S727)
  • 特异性A phospho specific peptide corresponding to residues surrounding Serine 727 of human Stat-1 was used as an immunogen. This antibody only detects Stat-1 phosphorylated at Serine 727.
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human STAT1 (phospho S727).

  • 阳性对照
    • HeLa cell lysate; Human stomach adenocarcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-STAT1 (phospho S727) antibody (BSA & Azide free) [EPR3146] (ab215820)

性能

应用

Our Abpromise guarantee covers the use of ab109461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 91 kDa (predicted molecular weight: 87 kDa).
IHC-P 1/100 - 1/250. Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
  • 应用说明Is unsuitable for ICC/IF.
  • 靶标

    • 功能Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
    • 疾病相关Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
      Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    • 序列相似性Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
    • 翻译后修饰Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
      Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
      ISGylated.
    • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Signal transducer and activator of transcription 1 91kD antibody
      • CANDF7 antibody
      • DKFZp686B04100 antibody
      • IMD31A antibody
      • IMD31B antibody
      • IMD31C antibody
      • ISGF 3 antibody
      • ISGF-3 antibody
      • OTTHUMP00000163552 antibody
      • OTTHUMP00000165046 antibody
      • OTTHUMP00000165047 antibody
      • OTTHUMP00000205845 antibody
      • Signal transducer and activator of transcription 1 91kDa antibody
      • Signal transducer and activator of transcription 1 antibody
      • Signal transducer and activator of transcription 1, 91kD antibody
      • Signal transducer and activator of transcription 1-alpha/beta antibody
      • STAT 1 antibody
      • Stat1 antibody
      • STAT1_HUMAN antibody
      • STAT91 antibody
      • Transcription factor ISGF 3 components p91 p84 antibody
      • Transcription factor ISGF-3 components p91/p84 antibody
      see all

    Anti-STAT1 (phospho S727) antibody [EPR3146] 图像

    • All lanes : Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/5000 dilution (purified)

      Lane 1 : Untreated HeLa whole cell lysate
      Lane 2 : HeLa whole cell lysate treated with etoposide
      Lane 3 : HeLa whole cell lysate treated with etoposide, followed by membrane treatment with phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 87 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 2% BSA/TBST
      Dilution buffer: 2% BSA/TBST

    • Immunohistochemical staining of paraffin embedded rat colon with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemical staining of paraffin embedded mouse colon with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemical staining of paraffin embedded human breast carcinoma with purified ab109461 at a working dilution of 1/200. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • All lanes : Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461) at 1/10000 dilution (unpurified)

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma)
      Lane 2 : HeLa (human cervix adenocarcinoma) membrane treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 dilution
      Developed using the ECL technique

      Predicted band size : 87 kDa
      Observed band size : 91 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      The lower section shows STAT1 detected with ab92506, anti-STAT1 antibody, to confirm that the same amount of lysate is used in each lane.

      Blocking and dilution biffer: 5% NFDM/TBST.

    • Unpurified ab109461, at a 1/100 dilution, staining STAT1 (phospho S727) in paraffin embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.

    Anti-STAT1 (phospho S727) antibody [EPR3146] (ab109461)参考文献

    This product has been referenced in:
    • Chen L  et al. Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression. Nat Commun 5:5241 (2014). WB . Read more (PubMed: 25348003) »
    • Camicia R  et al. BAL1/ARTD9 represses the anti-proliferative and pro-apoptotic IFN?-STAT1-IRF1-p53 axis in diffuse large B-cell lymphoma. J Cell Sci 126:1969-80 (2013). Read more (PubMed: 23487038) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116)
    Gel Running Conditions Reduced Denaturing
    Loading amount 10 µg
    Specification HCT116
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Chiara Vardabasso

    Verified customer

    提交于 Oct 27 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"