概述

  • 产品名称
    Anti-STAT1抗体[SM1]
    参阅全部 STAT1 一抗
  • 描述
    小鼠单克隆抗体[SM1] to STAT1
  • 特异性
    The antibody recognizes an epitope included within amino acids 721-733 of the 91 kDa STAT 1 protein.
  • 经测试应用
    适用于: IHC-P, IP, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    DNLLPMSPEEFDE

    , corresponding to amino acids 721-733 of STAT 1.

性能

应用

Our Abpromise guarantee covers the use of ab3987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 91 kDa (predicted molecular weight: 87 kDa).
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
  • 疾病相关
    Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
    Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • 序列相似性
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰
    Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
    Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
    ISGylated.
  • 细胞定位
    Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Signal transducer and activator of transcription 1 91kD antibody
    • CANDF7 antibody
    • DKFZp686B04100 antibody
    • IMD31A antibody
    • IMD31B antibody
    • IMD31C antibody
    • ISGF 3 antibody
    • ISGF-3 antibody
    • OTTHUMP00000163552 antibody
    • OTTHUMP00000165046 antibody
    • OTTHUMP00000165047 antibody
    • OTTHUMP00000205845 antibody
    • Signal transducer and activator of transcription 1 91kDa antibody
    • Signal transducer and activator of transcription 1 antibody
    • Signal transducer and activator of transcription 1, 91kD antibody
    • Signal transducer and activator of transcription 1-alpha/beta antibody
    • STAT 1 antibody
    • Stat1 antibody
    • STAT1_HUMAN antibody
    • STAT91 antibody
    • Transcription factor ISGF 3 components p91 p84 antibody
    • Transcription factor ISGF-3 components p91/p84 antibody
    see all

图片



  • Predicted band size : 87 kDa


    Induction of phosphorylation of STAT 1 at Ser727 in human malignant melanoma cells (short-term culture derived from a patient) in response to interferons. Subconfluent cells were serum-starved before exposure to activation dosages of IFN-gamma (10 ng/ml) and IFN-alpha (1000 IU/ml and 5000 IU/ml). Western blotting analysis of cell extracts shows detection of phosphorylated STAT-1 (Ser727) by the antibody PSM1 (upper panel) and total STAT 1 level by the antibody SM1 (lower panel).
  • Overlay histogram showing HeLa cells stained with ab3987 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3987, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

文献

This product has been referenced in:
  • Zhao W  et al. Lentivirus-mediated overexpression of CD97/ADGRE5 reverses dysregulated high glucose-induced endothelial cell migration. Mol Med Rep 15:3048-3054 (2017). WB ; Human . Read more (PubMed: 28358422) »
  • Iwata H  et al. PARP9 and PARP14 cross-regulate macrophage activation via STAT1 ADP-ribosylation. Nat Commun 7:12849 (2016). Read more (PubMed: 27796300) »

See all 5 Publications for this product

客户评价及客户问答

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
2e+006 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (primary human monocyte derived macrophages)
Specification
primary human monocyte derived macrophages
Treatment
100ug/ml MDP
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 26 2013

Application
Flow Cytometry
Fixation
BD #554722
Sample
Human Cell (primary human macrophages)
Specification
primary human macrophages
Gating Strategy
macrophages
Permeabilization
Yes - BD Phosflow #558050
Username

Abcam user community

Verified customer

提交于 Jul 15 2013

If you find the online order system too slow you can e-mail your order to orders@abcam.com or phone on 01223 472030 (dialing code +44) or fax your order on 01223 472038. Hope this helps.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册