Anti-STAT1 alpha抗体[EPYR2154] (ab92506)


  • 产品名称Anti-STAT1 alpha抗体[EPYR2154]
    参阅全部 STAT1 alpha 一抗
  • 描述
    兔单克隆抗体[EPYR2154] to STAT1 alpha
  • 特异性Based on Blast results using the immunogen sequence, this antibody should recognise the alpha but not the beta form (only 30% homology) of Stat1.
  • 经测试应用适用于: WB, IP, Flow Cytmore details
    不适用于: ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human STAT1 alpha aa 700 to the C-terminus.
    Database link: P42224

  • 阳性对照
    • HeLa, 293, NIH-3T3 and A431 cell lysates
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-STAT1 antibody (Alexa Fluor® 488) [EPYR2154] (ab193889)

    Anti-STAT1 antibody (Alexa Fluor® 647) [EPYR2154] (ab193890)

    Anti-STAT1 antibody (HRP) [EPYR2154] (ab193891)




Our Abpromise guarantee covers the use of ab92506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Predicted molecular weight: 87 kDa.
IP 1/50.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for ICC or IHC-P.
  • 靶标

    • 功能Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
    • 疾病相关Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
      Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
    • 序列相似性Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
    • 翻译后修饰Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
      Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
    • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CANDF7 antibody
      • DKFZp686B04100 antibody
      • ISGF 3 antibody
      • ISGF3 antibody
      • OTTHUMP00000163552 antibody
      • OTTHUMP00000165046 antibody
      • OTTHUMP00000165047 antibody
      • OTTHUMP00000205845 antibody
      • Signal transducer and activator of transcription 1 antibody
      • Signal transducer and activator of transcription 1, 91kDa antibody
      • Signal transducer and activator of transcription 1-alpha/beta antibody
      • Stat1 antibody
      • STAT1_HUMAN antibody
      • STAT91 antibody
      • Transcription factor ISGF-3 components p91/p84 antibody
      see all

    Anti-STAT1 alpha antibody [EPYR2154] 图像

    • All lanes : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/2000 dilution

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma) membrane
      Lane 2 : HeLa (human cervix adenocarcinoma) membrane treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 dilution
      developed using the ECL technique

      Predicted band size : 87 kDa

      Exposure time : 3 minutes

      Phospho STAT1 protein is revealed by ab109461, anti-STAT1 (phospho S727) antibody. ab92506 is used as pan control which detects total STAT1.

      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293 cell lysate
      Lane 3 : NIH-3T3 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 87 kDa
      Observed band size : 91 kDa (why is the actual band size different from the predicted?)

    Anti-STAT1 alpha antibody [EPYR2154] (ab92506)参考文献

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116)
    Gel Running Conditions Reduced Denaturing
    Loading amount 10 µg
    Specification HCT116
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    提交于 Aug 03 2015