概述

  • 产品名称Anti-STAT1 alpha抗体
    参阅全部 STAT1 alpha 一抗
  • 描述
    兔多克隆抗体to STAT1 alpha
  • 特异性No reaction to the 84 kDa STAT1b.
  • 经测试应用适用于: ICC/IF, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Peptide sequence corresponding to amino acids 712 to750 of human STAT1a (4). The sequences differ from themurine corresponding sequences by four amino acids.

  • 阳性对照
    • HeLa whole cell lysate, Jurkat whole cell lysate or A431 whole cell. lysate.
  • 常规说明JAK activated transcription.

性能

应用

Our Abpromise guarantee covers the use of ab2071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
IP Use a concentration of 2 - 4 µg/ml.
WB 1/1000 - 1/2000. Detects a band of approximately 200 kDa (predicted molecular weight: 100 kDa).

靶标

  • 功能Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
  • 疾病相关Note=STAT1 deficiency results in impaired immune response leading to severe mycobacterial and viral diseases. In the case of complete deficiency, patients can die of viral disease.
    Defects in STAT1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance.
  • 序列相似性Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • 翻译后修饰Phosphorylated on tyrosine and serine residues in response to IFN-alpha, IFN-gamma, PDGF and EGF. Phosphorylation on Tyr-701 (lacking in beta form) by JAK promotes dimerization and subsequent translocation to the nucleus. Phosphorylation on Ser-727 by several kinases including MAPK14, ERK1/2 and CAMKII on IFN-gamma stimulation, regulates STAT1 transcriptional activity. Phosphorylation on Ser-727 promotes sumoylation though increasing interaction with PIAS. Phosphorylation on Ser-727 by PKCdelta induces apoptosis in response to DNA-damaging agents.
    Sumoylated by SUMO1, SUMO2 and SUMO3. Sumoylation is enhanced by IFN-gamma-induced phosphorylation on Ser-727, and by interaction with PIAS proteins. Enhances the transactivation activity.
    ISGylated.
  • 细胞定位Cytoplasm. Nucleus. Translocated into the nucleus in response to IFN-gamma-induced tyrosine phosphorylation and dimerization.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CANDF7 antibody
    • DKFZp686B04100 antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • OTTHUMP00000163552 antibody
    • OTTHUMP00000165046 antibody
    • OTTHUMP00000165047 antibody
    • OTTHUMP00000205845 antibody
    • Signal transducer and activator of transcription 1 antibody
    • Signal transducer and activator of transcription 1, 91kDa antibody
    • Signal transducer and activator of transcription 1-alpha/beta antibody
    • Stat1 antibody
    • STAT1_HUMAN antibody
    • STAT91 antibody
    • Transcription factor ISGF-3 components p91/p84 antibody
    see all

Anti-STAT1 alpha antibody 图像

  • All lanes : Anti-STAT1 alpha antibody (ab2071) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : A431 whole cell lysate
    Lane 4 : K562 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate


    Predicted band size : 100 kDa
    Observed band size : 91 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry of STAT1a in HeLa cells with STAT1a antibody at 10 µg/ml.

Anti-STAT1 alpha antibody (ab2071)参考文献

This product has been referenced in:
  • Nagakura I  et al. STAT1 Regulates the Homeostatic Component of Visual Cortical Plasticity via an AMPA Receptor-Mediated Mechanism. J Neurosci 34:10256-63 (2014). Mouse . Read more (PubMed: 25080587) »
  • Carlos TS  et al. Parainfluenza virus 5 genomes are located in viral cytoplasmic bodies whilst the virus dismantles the interferon-induced antiviral state of cells. J Gen Virol 90:2147-56 (2009). ICC/IF ; Human . Read more (PubMed: 19458173) »

See all 2 Publications for this product

Product Wall

Unfortunately, this antibody has not been tested in gel supershift assays, therefore we are unable to comment on it's suitability.

Thank you for your interest in our products. Unfortunatly, none of our antibodies are phosphose specific. Regards, Steve Reed ProSci Inc.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"