概述

  • 产品名称
  • 描述
    兔多克隆抗体to StAR
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cow, Dog, Pig
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 15 - 238 of Human StAR (NP_000340).

  • 阳性对照
    • 293T, A431, HeLa, HepG2, MOLT4 and Raji cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab96637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.

靶标

  • 功能
    Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
  • 组织特异性
    Expressed in gonads, adrenal cortex and kidney.
  • 通路
    Steroid metabolism; cholesterol metabolism.
  • 疾病相关
    Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
  • 序列相似性
    Contains 1 START domain.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cholesterol trafficker antibody
    • Luteinizing hormone induced protein antibody
    • mitochondrial antibody
    • Mitochondrial steroid acute regulatory protein antibody
    • StAR antibody
    • StAR related lipid transfer (START) domain containing 1 antibody
    • STAR_HUMAN antibody
    • StARD1 antibody
    • START domain containing 1 antibody
    • START domain containing protein 1 antibody
    • START domain-containing protein 1 antibody
    • Steroid acute regulatory protein antibody
    • Steroidogenic acute regulator antibody
    • Steroidogenic acute regulatory protein antibody
    • Steroidogenic acute regulatory protein mitochondrial antibody
    see all

图片

  • Anti-StAR antibody (ab96637) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size : 32 kDa
  • All lanes : Anti-StAR antibody (ab96637) at 1/1000 dilution

    Lane 1 : Whole tissue lysate prepared from murine adrenal gland
    Lane 2 : Whole tissue lysate prepared from rat adrenal gland

    Lysates/proteins at 50 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 32 kDa


    Exposure time : 20 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

实验方案

文献

This product has been referenced in:
  • Fadhillah  et al. Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells. J Reprod Dev 63:75-85 (2017). WB ; Cow . Read more (PubMed: 27840375) »
  • Yang YL  et al. The role of GPR1 signaling in mice corpus luteum. J Endocrinol 230:55-65 (2016). IHC-P ; Mouse . Read more (PubMed: 27149986) »

See all 5 Publications for this product

客户评价及客户问答

Thank you very much for your interest in ab96637. Sinceab96637 has not been tested in pig, I can offer a discount off a future purchase if you buy ab96637 now, test it in pig, and submit feedback to us in the form of an Abreview. It doesn’t matte...

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Thank you for submitting your completed questionnaire. To our knowledge ab96637 has not been previously tested in IHC-P. Thus is may be that this antibody does not function effectively in this application. However, results may be improved by doing an ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Adrenal)
Loading amount
50 µg
Specification
Adrenal
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Aug 23 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Oct 20 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1)
Loading amount
25 µg
Specification
THP-1
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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