概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to StAR
  • 经测试应用
    适用于: WB, ELISA, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment: EAMQKALGIL SNQEGWKKES QQDNGDKVMS KVVPDVGKVF RLEVVVDQPM ERLYEELVER MEAMGEWNPN VKEIKVLQKI GKDTFITHEL AAEAAGNLVG , corresponding to amino acids 81-180 of Human StAR

应用

Our Abpromise guarantee covers the use of ab58013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 21094146

靶标

  • 功能
    Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.
  • 组织特异性
    Expressed in gonads, adrenal cortex and kidney.
  • 通路
    Steroid metabolism; cholesterol metabolism.
  • 疾病相关
    Defects in STAR are the cause of adrenal hyperplasia type 1 (AH1) [MIM:201710]. The most severe form of adrenal hyperplasia. It is a condition characterized by onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. Affected individuals are phenotypic females irrespective of gonadal sex, and frequently die in infancy if mineralocorticoid and glucocorticoid replacement are not instituted.
  • 序列相似性
    Contains 1 START domain.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cholesterol trafficker antibody
    • Luteinizing hormone induced protein antibody
    • mitochondrial antibody
    • Mitochondrial steroid acute regulatory protein antibody
    • StAR antibody
    • StAR related lipid transfer (START) domain containing 1 antibody
    • STAR_HUMAN antibody
    • StARD1 antibody
    • START domain containing 1 antibody
    • START domain containing protein 1 antibody
    • START domain-containing protein 1 antibody
    • Steroid acute regulatory protein antibody
    • Steroidogenic acute regulator antibody
    • Steroidogenic acute regulatory protein antibody
    • Steroidogenic acute regulatory protein mitochondrial antibody
    see all

Anti-StAR antibody 图像

  • Anti-StAR antibody (ab58013) at 1/1000 dilution + Mouse liver lysates at 50 µg

    Secondary
    HRP-conjugated Goat polyclonal to mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 27,30 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    Liver lysates prepared from mouse injected with StarD1 adenovirus expressing recombinant StarD1 protein.

    Primary diluted in TBST + 2.5% milk and incubated with sample for 12 hours at 4°C.

  • Anti-StAR antibody (ab58013) at 1/1000 dilution (in TBST + 2.5% milk for 16 hours at 4°C) + Whole cell lysate of human THP1 cells at 50 µg

    Secondary
    An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 30 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 4 hours at 22°C

    See Abreview

Anti-StAR antibody (ab58013)参考文献

This product has been referenced in:
  • Xiong S  et al. Effects of luteinizing hormone receptor signaling in prostate cancer cells. Prostate 75:141-50 (2015). Read more (PubMed: 25393079) »
  • França MM  et al. POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells. Braz J Med Biol Res 48:1087-94 (2015). WB . Read more (PubMed: 26421867) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human adrenal corticocarcinoma H295R cells)
Permeabilization
Yes - 0.5% TX100
Specification
Human adrenal corticocarcinoma H295R cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Jun 27 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Human adrenal corticocarcinoma H295R cells)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Human adrenal corticocarcinoma H295R cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Jun 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse testis)
Permeabilization
Yes - 0.5% TX100
Specification
mouse testis
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Dec 02 2016

Thank you for contacting us.

This is not a phopspho specific antibody which means this antibody would detect total StAR protein i.e. phospho as well as non-phospho form. However it will not be able to distinguish between the two forms.
Read More

Thank you for contacting Abcam regarding ab58013. I am sorry that you have been experiencing difficulties with this antibody in WB.  I have reviewed the protocol information provided and would like to make some suggestions to improve your results wi...

Read More

Indeed there are two entries in the Uniprot database, Q6IBK0 and P49675. Both are for the same protein. However the entry which is best to refer to is the P49675 as this is the entry which is manually annotated and curated (Swissprot). Indeed, Uniprot ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1 cells)
Loading amount
50 µg
Specification
THP-1 cells
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Sep 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (testis)
Loading amount
35 µg
Specification
testis
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Feb 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver)
Loading amount
50 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Nov 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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