Anti-Staphylococcus aureus抗体[704] (ab37644)


  • 产品名称Anti-Staphylococcus aureus抗体[704]
    参阅全部 Staphylococcus aureus 一抗
  • 描述
    小鼠单克隆抗体[704] to Staphylococcus aureus
  • 经测试应用适用于: ELISA, ICC/IF, IHC-P, Flow Cytmore details
  • 种属反应性
    Reacts with Staphylococcus aureus, Protein A-negative Staphylococcus aureus and Staphylococcus epidermidis. Does not cross-react with Corynebacteria species, Streptococcus species or Mycoplasma species.
  • 免疫原

    UV-inactivated Staphylococcus aureus cells.



Our Abpromise guarantee covers the use of ab37644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration. May be used to detect and quantify Staphylococcus aureus cells.
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21208278
Flow Cyt Use at an assay dependent concentration. PubMed: 20016671ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.


  • 相关性S.aureus binds IgG molecules by their Fc region. In serum, the bacteria will bind IgG molecules in the wrong orientation on their surface, which hinders opsonization and phagocytosis. Mutants of S. aureus lacking protein A are more efficiently phagocytosed in vitro, and mutants in infection models have diminished virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
  • 细胞定位Secreted; cell wall.
  • 别名
    • IgG binding protein A antibody
    • Immunoglobulin G binding protein A antibody
    • SPA antibody

Anti-Staphylococcus aureus antibody [704] 图像

  • ab37644 staining Staphylococcus aureus in 16HBE cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with 4% paraformaldehyde, blocked with 5% normal donkey serum, and stained with ab37644. The secondary used was an anti-mouse IgG Alexa Fluor 488 (green). Phalloidin (blue).

    Scale bar 20 µm.

Anti-Staphylococcus aureus antibody [704] (ab37644)参考文献

This product has been referenced in:
  • Chen J  et al. [(99m)Tc]cFLFLF for Early Diagnosis and Therapeutic Evaluation in a Rat Model of Acute Osteomyelitis. Mol Imaging Biol 17:337-44 (2015). Read more (PubMed: 25323104) »
  • Johansen LK  et al. A porcine model of acute, haematogenous, localized osteomyelitis due to Staphylococcus aureus: a pathomorphological study. APMIS 119:111-8 (2011). IHC-P . Read more (PubMed: 21208278) »

See all 5 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Mouse Cultured Cells (Mast Cells (HMC-1))
Permeabilization Yes - Triton X-100
Specification Mast Cells (HMC-1)
Blocking step BSA as blocking agent for 60 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Formaldehyde

Dr. Tim Biggs

Verified customer

提交于 Jun 15 2016

Ab20002 - Mouse monoclonal [702] to Staphylococcus aureus and Ab37644 - Mouse monoclonal [704] to Staphylococcus aureus recognize peptidoglycan which is a cell wall component, so, by definition, this is on the surface of the cells.

About the...

Read More

Thank you for your enquiry.

I havecontacted the source for all the Staphylococcus aureus antibodies we have that have been tested in WB and/or ELISA to ask if they have been tested for crossreactivity. Here is the information:


Read More

Thank you for contacting Abcam

The antibody, ab37644 is covered under our Abpromise for six months and is guaranteed to work in ICC/IF. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a repl...

Read More

Thank you for contacting Abcam.

The antibody ab37644 recognizes an epitope on the peptidoglycan. The epitope recognized is not on Protein A.

I hope that this helps clarify matters. If there is anything else I can help you with, plea...

Read More

Thank you for calling Abcam earlier.

I have attached our protocol for mouse on mouse staining and the link to our EasyLink kits is below:

Read More

Thank you for contacting us. Staph aureus ab37644, recognizes an epitope on the peptidoglycan. A more detailed location has not been mapped. The immunogen is UV-inactivated Staphylococcus aureus cells. I hope this information is helpful to you. Pl...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (THP-1 Macrophages)
Specification THP-1 Macrophages
Fixative No fixative - LIVE cells
Permeabilization No

Abcam user community

Verified customer

提交于 Jul 26 2007