The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/300. This dilution will give an absorbance of >1.0 for a 10 ng/ml standard after a five minute incubation.
WB: 1/1000. Predicted molecular weight: 35.9 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Staphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity.