Anti-Staphylococcus alpha Hemolysin抗体(Biotin) (ab15950)

概述

  • 产品名称Anti-Staphylococcus alpha Hemolysin抗体(Biotin)
    参阅全部 Staphylococcus alpha Hemolysin 一抗
  • 描述
    羊多克隆抗体to Staphylococcus alpha Hemolysin (Biotin)
  • 偶联物Biotin
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Staphylococcus aureus
  • 免疫原

    Highly pure (> 95%) Staphylococcus aureus alpha Hemolysin alpha

性能

应用

Our Abpromise guarantee covers the use of ab15950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/2000. This dilution of antibody will give an absorbance of > 1.0 for a 10ng standard after a 5 minute incubation.
WB 1/1000. Predicted molecular weight: 35.9 kDa.

靶标

  • 相关性Staphylococcus aureus alpha Hemolysin binds to the membrane of eukaryotic cells resulting in the release of low-molecular weight molecules and leading to an eventual osmotic lysis. The toxin self-assembles to form first, a nonlytic oligomeric intermediate, and then, a mushroom-shaped homoheptamer structure. Heptamer oligomerization and pore formation is required for lytic activity.
  • 细胞定位Secreted
  • 数据库链接
    • 别名
      • Alpha hemolysin antibody
      • Alpha HL antibody
      • Alpha toxin antibody
      • Hla antibody
      • Hly antibody
      • Staphylococcus alpha HL antibody
      • Staphylococcus alpha toxin antibody
      see all

    Anti-Staphylococcus alpha Hemolysin antibody (Biotin) 图像

    • All lanes : Anti-Staphylococcus alpha Hemolysin antibody (Biotin) (ab15950) at 1/1000 dilution

      Lane 1 : Positive control (purified Hla (100ng/ml))
      Lane 2 : Staph. aureus infected skin from patient with atopic dermatitis - untreated
      Lane 3 : Staph. aureus infected skin from patient with atopic dermatitis - treated with HBD2 (0,2µg/ml)
      Lane 4 : Staph. aureus infected skin from patient with atopic dermatitis - treated with HBD2 (2,0µg/ml)
      Lane 5 : Staph. aureus infected skin from patient with atopic dermatitis - treated with HBD2 (20µg/ml)
      Lane 6 : Staph. aureus infected skin from patient with atopic dermatitis - treated with penicillin (50ng/ml)

      Lysates/proteins at 15 µg per lane.


      Predicted band size : 35.9 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

      Image courtesy of an anonymous Abreview.

      See Abreview

    Anti-Staphylococcus alpha Hemolysin antibody (Biotin) (ab15950)参考文献

    ab15950 has not yet been referenced specifically in any publications.

    Product Wall

    Application ELISA
    Sample Staphylococcus aureus Cell (different dilutions of S. aureus aplha toxin)
    Specification different dilutions of S. aureus aplha toxin
    Type Sandwich (Detection)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 22 2013

    I can understand that you are disappointed. I am sorry for this wrong information that was cited on the datasheet. I am pleased to provide another vial of the ab15950 for you.


    I have issued a free of charge replacement with the order numb...

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    Thank you very much for your patience. I am sorry that you experiencing problems with this two antibodies.

    I am still waiting for some QC results from the lab and I will forward this to you as soon as I recive the answer.

    Every appli...

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    Thank you for contacting us.

    In order to help you with these antibodies I would like to get some more information about the experiment.

    1. Do I understand it right that the performed experiment is a sandwich ELISA? Do you mean the ab1...

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    Thank you for contacting us. I have spoken with the lab regarding your questions.

    I am sorry to say I have no definite answers to any of these questions. For the third question, I have a probable answer; as the antiserum is a polyclonal the ...

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    Thank you for your response.

    Many of the questions seem to be about the alpha Hemolysin protein.Just to be sure, I would like clarify that this product, ab15950 Anti-Staphylococcus alpha Hemolysin antibody (Biotin), is an antibody raised in ...

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    Thank you for contacting us.

    The productab15950 Anti-Staphylococcus alpha Hemolysin antibody (Biotin) has been conjugated with biotin and will be recognized by streptavidin.

    I hope this information is helpful to you. Please do...

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    Application Western blot
    Sample Human Tissue lysate - whole (skin biopsie)
    Loading amount 15 µg
    Specification skin biopsie
    Treatment see below
    Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"