概述

  • 产品名称
    Anti-Stanniocalcin 1抗体
    参阅全部 Stanniocalcin 1 一抗
  • 描述
    兔多克隆抗体to Stanniocalcin 1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (LQNSAVLLVL VISASATHEA EQNDSVSPRK SRVAAQNSAE VVRCLNSALQ) of Human Stanniocalcin 1 (NP_003146).

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Stanniocalcin 1 antibody 图像

  • Anti-Stanniocalcin 1 antibody (ab83065) at 1 µg/ml (5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

实验方案

Anti-Stanniocalcin 1 antibody (ab83065)参考文献

This product has been referenced in:
  • Xiang J  et al. Regulation of Intestinal Epithelial Calcium Transport Proteins by Stanniocalcin-1 in Caco2 Cells. Int J Mol Sci 17:N/A (2016). Read more (PubMed: 27409607) »
  • Waszak P  et al. Preconditioning enhances the paracrine effect of mesenchymal stem cells in preventing oxygen-induced neonatal lung injury in rats. Stem Cells Dev 21:2789-97 (2012). WB ; Rat . Read more (PubMed: 22533467) »

See all 2 Publications for this product

Product Wall

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (293T)
Specification
293T
Treatment
transiently transfected
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Eric Routh

Verified customer

提交于 Mar 26 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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