Anti-STAM2 (phospho Y192)抗体(ab63569)

概述

  • 产品名称Anti-STAM2 (phospho Y192)抗体
    参阅全部 STAM2 一抗
  • 描述
    兔多克隆抗体to STAM2 (phospho Y192)
  • 特异性ab63569 detects endogenous levels of STAM2 only when phosphorylated at tyrosine 192.
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthesized phosphopeptide derived from human STAM2 around the phosphorylation site of tyrosine 192(S-L-YP-P-S).

  • 阳性对照
    • NIH/3T3 cytoplasmic lysate IHC-P: Human lung FFPE tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab63569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

靶标

  • 功能Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the STAM family.
    Contains 1 ITAM domain.
    Contains 1 SH3 domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • 结构域The VHS and UIM domains mediate the interaction with ubiquitinated proteins.
    The SH3 domain mediates the interaction with USP8.
    Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
  • 翻译后修饰Phosphorylated in response to IL-2, GM-CSF, EGF and PDGF.
  • 细胞定位Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp564C047 antibody
    • Hbp antibody
    • Hrs-binding protein antibody
    • HSE1 homolog antibody
    • Signal transducing adapter molecule 2 antibody
    • Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 antibody
    • Signal transducing adaptor molecule 2A antibody
    • Signal transducing adaptor molecule 2B antibody
    • Signal transducing adaptor molecule antibody
    • STAM like protein containing SH3 and ITAM domains 2 antibody
    • STAM-2 antibody
    • Stam2 antibody
    • STAM2_HUMAN antibody
    • STAM2A antibody
    • STAM2B antibody
    see all

Anti-STAM2 (phospho Y192) antibody 图像

  • IHC image of STAM2 (phospho Y192) staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63569, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-STAM2 (phospho Y192) antibody (ab63569) at 1/500 dilution

    Lane 1 : Extracts from NIH/3T3 cells (5-30µg), treated with EGF (200ng/ml, 30mins), minus immunising peptide.
    Lane 2 : Extracts from NIH/3T3 cells (5-30µg), treated with EGF (200ng/ml, 30mins), plus immunising peptide (5-10µg).


    Predicted band size : 58 kDa
    Observed band size : 58 kDa

Anti-STAM2 (phospho Y192) antibody (ab63569)参考文献

ab63569 has not yet been referenced specifically in any publications.

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