概述

  • 产品名称Anti-STAM1抗体
    参阅全部 STAM1 一抗
  • 描述
    兔多克隆抗体to STAM1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 300-350 of Human STAM1 according to NP_003464.1.

  • 阳性对照
    • Whole cell lysate from HeLa, 293T and Jurkat cells.

性能

应用

Our Abpromise guarantee covers the use of ab117813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

  • 功能Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the STAM family.
    Contains 1 ITAM domain.
    Contains 1 SH3 domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • 翻译后修饰Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.
  • 细胞定位Cytoplasm. Early endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HSE1 homolog antibody
    • Signal transducing adapter molecule 1 antibody
    • signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 antibody
    • STAM antibody
    • STAM-1 antibody
    • STAM1_HUMAN antibody
    see all

Anti-STAM1 antibody 图像

  • All lanes : Anti-STAM1 antibody (ab117813) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from 293T cells at 50 µg
    Lane 4 : Whole cell lysate from Jurkat cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 58 kDa


    Exposure time : 30 seconds
  • ab117813 at 1 µg/ml staining STAM1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
    Lane 1; IP using ab117813 at 6 µg/mg lysate.
    Lane 1; IP using an antibody to a downstream epitope.
    Lane 2; IP using an antibody to an alternative downstream epitope.
    Lane 3; IP using control IgG.
    1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.

Anti-STAM1 antibody (ab117813)参考文献

ab117813 has not yet been referenced specifically in any publications.

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