Anti-STAM1抗体(ab117813)
Key features and details
- Rabbit polyclonal to STAM1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-STAM1抗体
参阅全部 STAM1 一抗 -
描述
兔多克隆抗体to STAM1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide, corresponding to a region within amino acids 300-350 of Human STAM1 according to NP_003464.1.
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阳性对照
- Whole cell lysate from HeLa, 293T and Jurkat cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117813于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 58 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 58 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the STAM family.
Contains 1 ITAM domain.
Contains 1 SH3 domain.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain. -
翻译后修饰
Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB. -
细胞定位
Cytoplasm. Early endosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 512734 Cow
- Entrez Gene: 8027 Human
- Omim: 601899 Human
- SwissProt: Q92783 Human
- Unigene: 335391 Human
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别名
- DKFZp686J2352 antibody
- HSE1 homolog antibody
- OTTHUMP00000019237 antibody
see all
图片
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All lanes : Anti-STAM1 antibody (ab117813) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 30 seconds -
ab117813 at 1 µg/ml staining STAM1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab117813 at 6 µg/mg lysate.
Lane 1; IP using an antibody to a downstream epitope.
Lane 2; IP using an antibody to an alternative downstream epitope.
Lane 3; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised ECL with a 10 second exposure.
数据表及文件
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Datasheet download
文献 (0)
ab117813 尚未被引用在任何文献中。