antigen sequence: THQFPELMGV FAKAMIDIFC SAEFRDWNCK SIFMRVEDEL EIPPAPQSQH FQ, corresponding to amino acids 533-584 of Human ST7.
- Human adrenal gland tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
May act as a tumor suppressor.
Ubiquitously expressed, with highest levels in heart, liver and pancreas.
Belongs to the ST7 family.
Information by UniProt
- ETS7q antibody
- FAM4A antibody
- FAM4A1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ST7 antibody (ab122460)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122460 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST7 antibody (ab122460)
ab122460, at 1/500 dilution, staining ST7 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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