antigen sequence: PQARISAAHE ALEINEIRSR VEVPLIASST IWEIKLLPKC ATAY, corresponding to amino acids of Human ST7.
- RT 4 and U 251 cell line lysates; Human heart muscle tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/250 - 1/500.
||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May act as a tumor suppressor.
Ubiquitously expressed, with highest levels in heart, liver and pancreas.
Belongs to the ST7 family.
Information by UniProt
- ETS7q antibody
- FAM4A antibody
- FAM4A1 antibody
Immunocytochemistry/ Immunofluorescence - Anti-ST7 antibody (ab122459)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122459 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-ST7 antibody (ab122459)
All lanes : Anti-ST7 antibody (ab122459) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST7 antibody (ab122459)
ab122459, at 1/500, staining ST7 in paraffin embedded Human heart muscle tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-ST7 antibody(ab122459)
Immunohistochemical staining of Human pancreas shows moderate membranous positivity using ab122459
has not yet been referenced specifically in any publications.
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