Anti-ST6GALNAC5抗体(ab69855)

概述

  • 产品名称
    Anti-ST6GALNAC5抗体
    参阅全部 ST6GALNAC5 一抗
  • 描述
    兔多克隆抗体to ST6GALNAC5
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cat, Dog, Pig, Chimpanzee, Zebrafish
  • 免疫原

    Synthetic peptide: KGQVYNNLHL LSQVLPRLKA FMITRHKMLQ FDELFKQETG KDRKISNTWL, corresponding to amino acids 182-231 of Human ST6GALNAC5

  • 阳性对照
    • Jurkat cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab69855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Involved in the biosynthesis of ganglioside GD1a from GM1b. It exhibits higher activity with glycolipids than with glycoproteins.
  • 通路
    Protein modification; protein glycosylation.
  • 序列相似性
    Belongs to the glycosyltransferase 29 family.
  • 细胞定位
    Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6-sialyltransferase 5 antibody
    • 6-sialyltransferase V antibody
    • Alpha N acetylgalactosaminide alpha 2,6 sialyltransferase 5 antibody
    • Alpha-N-acetylgalactosaminide alpha-2 antibody
    • GalNAc alpha 2,6 sialyltransferase V antibody
    • GalNAc alpha-2 antibody
    • GD1 alpha synthase antibody
    • SIA7E_HUMAN antibody
    • Sialyltransferase 7E antibody
    • Sialyltransferase E antibody
    • SIAT7 E antibody
    • SIAT7-E antibody
    • SIAT7E antibody
    • ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 antibody
    • ST6GalNAc V antibody
    • St6galnac5 antibody
    • ST6GalNAcV antibody
    see all

Anti-ST6GALNAC5 antibody 图像

  • Anti-ST6GALNAC5 antibody (ab69855) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 39 kDa
    Observed band size : 39 kDa
  • Anti-ST6GALNAC5 antibody (ab69855) at 1/5000 dilution (in PBS + 0.1% Tween + 5% milk powder for 16 hours at 4°C) + Whole cell lysate of Human WT MDA MB231 cells at 20 µg

    Secondary
    An HRP-polymer Goat anti-rabbit polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Predicted band size : 39 kDa
    Observed band size : 39 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

实验方案

Anti-ST6GALNAC5 antibody (ab69855)参考文献

ab69855 has not yet been referenced specifically in any publications.

Product Wall

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (WT MDA MB231)
Loading amount
20 µg
Specification
WT MDA MB231
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 09 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Lewis lung carcinoma)
Loading amount
10 µg
Specification
Lewis lung carcinoma
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

提交于 Aug 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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