Anti-ST6GALNAC1抗体(ab82821)

概述

  • 产品名称Anti-ST6GALNAC1抗体
    参阅全部 ST6GALNAC1 一抗
  • 描述
    兔多克隆抗体to ST6GALNAC1
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 396-445 (LIKGYEQDVG TRTSFYGFTA FSLTQSLLIL GNRGFKNVPL GKDVRYLHFL) of human ST6GALNAC1 (NP_060884).

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab82821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

靶标

  • 通路Protein modification; protein glycosylation.
  • 序列相似性Belongs to the glycosyltransferase 29 family.
  • 细胞定位Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6-sialyltransferase 1 antibody
    • 6-sialyltransferase I antibody
    • Alpha N acetylgalactosaminide alpha 2 6 sialyltransferase 1 antibody
    • Alpha N acetylgalactosaminide alpha 2 6 sialyltransferase antibody
    • Alpha-N-acetylgalactosaminide alpha-2 antibody
    • GalNAc alpha 2 6 sialyltransferase I antibody
    • GalNAc alpha-2 antibody
    • HSY11339 antibody
    • SIA7A_HUMAN antibody
    • Sialyltransferase 7A antibody
    • SIAT7-A antibody
    • SIAT7A antibody
    • ST6 (alpha N acetyl neuraminyl 2 3 beta galactosyl 1 3) N acetylgalactosaminide alpha 2 6 sialyltransferase 1 antibody
    • ST6GalNAc I antibody
    • ST6GALNAC1 antibody
    • ST6GalNAcI antibody
    • STYI antibody
    see all

Anti-ST6GALNAC1 antibody 图像

  • Anti-ST6GALNAC1 antibody (ab82821) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 69 kDa
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab92821 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92821, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ST6GALNAC1 antibody (ab82821)参考文献

ab82821 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Lewis Lung Carcinoma)
Loading amount 10 µg
Specification Lewis Lung Carcinoma
Gel Running Conditions Non-reduced Non-Denaturing (Native) (4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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提交于 Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"