概述

  • 产品名称
    Anti-ST3GAL5抗体
    参阅全部 ST3GAL5 一抗
  • 描述
    兔多克隆抗体to ST3GAL5
  • 经测试应用
    适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Cow, Cat, Dog, Pig, Chimpanzee
  • 免疫原

    Synthetic peptide: VQKAPKDSEA ESKYDPPFGF RKFSSKVQTL LELLPEHDLP EHLKAKTCRR, corresponding to a region within N terminal amino acids 145-194 of Human ST3GAL5

  • 阳性对照
    • Jurkat cell lysate and human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab65541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

靶标

  • 功能
    Catalyzes the formation of ganglioside GM3 (alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1, 4-beta-D-glucosylceramide).
  • 组织特异性
    Ubiquitous. High expression in brain, skeletal muscle, placenta, and testis.
  • 疾病相关
    Defects in ST3GAL5 are the cause of Amish infantile epilepsy syndrome (AIES) [MIM:609056]. AIES is an autosomal recessive, infantile-onset symptomatic epilepsy associated with developmental stagnation and blindness.
  • 序列相似性
    Belongs to the glycosyltransferase 29 family.
  • 翻译后修饰
    N-glycosylated.
  • 细胞定位
    Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-sialyltransferase antibody
    • CMP-NeuAc:lactosylceramide alpha-2 antibody
    • CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase antibody
    • EC 2.4.99.9 antibody
    • Ganglioside GM3 synthase antibody
    • Lactosylceramide alpha-2,3-sialyltransferase antibody
    • Sialyltransferase 9 antibody
    • SIAT9 antibody
    • SIAT9_HUMAN antibody
    • SIATGM3S antibody
    • ST3 beta-galactoside alpha-2,3-sialyltransferase 5 antibody
    • ST3GAL antibody
    • ST3Gal V antibody
    • ST3GAL5 antibody
    • ST3GalV antibody
    see all

图片

  • Anti-ST3GAL5 antibody (ab65541) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab65541 at 4µg/ml staining ST3GAL5 in epithelial cells of human kidney renal tubule (indicated by arrows) by Immunohistochemistry, Paraffin-embedded tissue.

文献

ab65541 has not yet been referenced specifically in any publications.

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