Anti-ST3GAL4抗体(ab87114)

概述

  • 产品名称
    Anti-ST3GAL4抗体
  • 描述
    兔多克隆抗体to ST3GAL4
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Chimpanzee
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 252-301 (KQKPTTGLLA ITLALHLCDL VHIAGFGYPD AYNKKQTIHY YEQITLKSMA) of Human ST3GAL4 (NP_006269).

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab87114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    ST3GAL4 belongs to the glycosyltransferase 29 family. It may catalyze the formation of the NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc-sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. It may also be involved in the biosynthesis of the sialyl Lewis X determinant. ST3GAL4 is highly expressed in adult placenta, ovary and testes. There are five named isoforms.
  • 细胞定位
    Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein and secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • 数据库链接
  • 别名
    • Alpha 2 3 sialyltransferase IV antibody
    • Alpha 2 3 ST antibody
    • Alpha 3 N acetylneuraminyltransferase antibody
    • Beta galactoside alpha 2 3 sialyltransferase antibody
    • CGS23 antibody
    • CMP N acetylneuraminate beta galactosamide alpha 2 3 sialyltransferase antibody
    • FLJ11867 antibody
    • FLJ46764 antibody
    • Gal NAc6S antibody
    • NANTA3 antibody
    • SAT 3 antibody
    • SAT3 antibody
    • Sialyltransferase 4C (beta galactosidase alpha 2 3 sialytransferase) antibody
    • Sialyltransferase 4C (beta galactoside alpha 2 3 sialytransferase) antibody
    • SIAT4 antibody
    • SIAT4 C antibody
    • SIAT4C antibody
    • ST 4 antibody
    • ST3 beta galactoside alpha 2 3 sialyltransferase 4 antibody
    • ST3Gal III antibody
    • ST3GalIV antibody
    • ST4 antibody
    • STZ antibody
    see all

图片

  • Anti-ST3GAL4 antibody (ab87114) at 1 µg/ml + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : ~38 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab87114 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Lewis Lung Carcinoma cells (LLC1))
Loading amount
10 µg
Specification
Lewis Lung Carcinoma cells (LLC1)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Username

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提交于 Aug 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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