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Our Abpromise guarantee covers the use of ab86563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 115 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ICC/IF||Use at an assay dependent concentration.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cerebellum tissue labelling ST18 with ab86563 at 5.0µg/ml.
Image courtesy of Stephan Bartholomä by Abreview.ab86563 used in Western Blot at a 1/1000 dilution. Whole cell lysate was prepared from leukemic cell lines and 5µg was added to each lane. A goat anti-rabbit polyclonal was used at a 1/10,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"