概述

  • 产品名称
    Anti-ST18抗体
    参阅全部 ST18 一抗
  • 描述
    兔多克隆抗体to ST18
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 900-949 (IKKGKVSEEL MTIKLKATGG IESDEEIRHL DEEIKELNES NLKIEADMMK) of Human ST18 (NP_055497)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Hela cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab86563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 115 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells.
  • 组织特异性
    Detected at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Detected at even lower levels in mammary epithelial cells and breast cancer cells.
  • 序列相似性
    Contains 6 C2HC-type zinc fingers.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • KIAA0535 antibody
    • St18 antibody
    • ST18_HUMAN antibody
    • suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein) antibody
    • Suppression of tumorigenicity 18 protein antibody
    • Suppression of tumorigenicity protein 18 antibody
    • Zinc finger protein 387 antibody
    • Znf387 antibody
    see all

图片

  • Western blot - Anti-ST18 antibody (ab86563)
    Western blot - Anti-ST18 antibody (ab86563)
    Anti-ST18 antibody (ab86563) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 115 kDa



    Gel concentration: 6-18%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST18 antibody (ab86563)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ST18 antibody (ab86563)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain, cerebellum tissue labelling ST18 with ab86563 at 5.0µg/ml.

  • Western blot - Anti-ST18 antibody (ab86563)
    Western blot - Anti-ST18 antibody (ab86563)Image courtesy of Stephan Bartholomä by Abreview.
    ab86563 used in Western Blot at a 1/1000 dilution. Whole cell lysate was prepared from leukemic cell lines and 5µg was added to each lane. A goat anti-rabbit polyclonal was used at a 1/10,000 dilution.

文献

This product has been referenced in:
  • Shukla R  et al. Endogenous retrotransposition activates oncogenic pathways in hepatocellular carcinoma. Cell 153:101-11 (2013). IHC, ChIP . Read more (PubMed: 23540693) »

See 1 Publication for this product

发表研究结果有使用 ab86563？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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3 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Frozen sections) abreview for Anti-ST18 antibody

 Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton
Fixative
Paraformaldehyde
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Oct 28 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-ST18 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Acute myeloide leukemic cell line KG-1)
Specification
Acute myeloide leukemic cell line KG-1
Fixative
Formaldehyde
Permeabilization
Yes - Triton X 0,25%
Blocking step
PBS with FCS (10%) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Read More
abreview image
Username

Mr. Stephan Bartholomä

Verified customer

提交于 Jul 20 2011

Western blot abreview for Anti-ST18 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (leukemic cell lines)
Loading amount
5 µg
Specification
leukemic cell lines
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 20°C
Read More
abreview image
Username

Mr. Stephan Bartholomä

Verified customer

提交于 Apr 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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