概述
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产品名称Anti-SSRP1抗体
参阅全部 SSRP1 一抗 -
描述兔多克隆抗体to SSRP1
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经测试应用适用于: WB, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow, Zebrafish
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免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 75 and 308 of Human SSRP1 (NP_003137)
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阳性对照
- Molt-4 whole cell lysate and Cal27 xenograft tissue
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0 -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab96704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 81 kDa. | |
| IHC-P | 1/100 - 1/250. |
靶标
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功能Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
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序列相似性Belongs to the SSRP1 family.
Contains 1 HMG box DNA-binding domain. -
结构域The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
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翻译后修饰Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
Sumoylated. -
细胞定位Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
- Information by UniProt
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数据库链接
- Entrez Gene: 282082 Cow
- Entrez Gene: 100413716 Human
- Entrez Gene: 6749 Human
- Entrez Gene: 20833 Mouse
- Entrez Gene: 81785 Rat
- Entrez Gene: 386844 Zebrafish
- Omim: 604328 Human
- SwissProt: Q08945 Human
see all -
别名
- Chromatin-specific transcription elongation factor 80 kDa subunit antibody
- Cisplatin DNA SSRP antibody
- Facilitates chromatin remodeling 80 kDa subunit antibody
see all
Anti-SSRP1 antibody 图像
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Western blot - SSRP1 antibody (ab96704)Anti-SSRP1 antibody (ab96704) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg
Predicted band size : 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SSRP1 antibody (ab96704)ab96704, at a 1/100 dilution, staining human SSRP1 in Cal27 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Anti-SSRP1 antibody (ab96704)参考文献
ab96704 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"