概述

  • 产品名称Anti-SSRP1抗体[3E4]
    参阅全部 SSRP1 一抗
  • 描述
    小鼠单克隆抗体[3E4] to SSRP1
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment (Human)

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab26229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.
IP 1/200.

靶标

  • 功能Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
  • 序列相似性Belongs to the SSRP1 family.
    Contains 1 HMG box DNA-binding domain.
  • 结构域The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
  • 翻译后修饰Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
    Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
    Sumoylated.
  • 细胞定位Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chromatin-specific transcription elongation factor 80 kDa subunit antibody
    • Cisplatin DNA SSRP antibody
    • Facilitates chromatin remodeling 80 kDa subunit antibody
    • Facilitates chromatin transcription complex 80 kDa subunit antibody
    • Facilitates chromatin transcription complex subunit SSRP1 antibody
    • FACT 80 kDa subunit antibody
    • FACT complex subunit SSRP1 antibody
    • FACT80 antibody
    • FACTp80 antibody
    • hSSRP1 antibody
    • Recombination signal sequence recognition protein 1 antibody
    • Recombination signal sequence recognition protein antibody
    • SSRP 1 antibody
    • SSRP1 antibody
    • SSRP1_HUMAN antibody
    • Structure specific recognition protein 1 antibody
    • Structure-specific recognition protein 1 antibody
    • T160 antibody
    see all

Anti-SSRP1 antibody [3E4] 图像

  • Anti-SSRP1 antibody [3E4] (ab26229) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7898)
    Developed using the ECL technique

    Predicted band size : 81 kDa
  • 2.5 mcg of SSRP1 were immobilized on 50 mcL of anti-mouse Dynabeads prior to IP. Nuclear lysate from HeLa cells treated with Benzonase was loaded at 1 x 107 cells. ab26229 used at a 1/200 dilution for IP. After IP the beads were washed three times with TBS + 0.5%NP40 + 5mM EDTA. ab26229 used at 1/1000 dilution for WB.

    See Abreview

Anti-SSRP1 antibody [3E4] (ab26229)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - nuclear (HeLa nuclei treated with benzonase)
Total protein in input 1e+007 cells
Specification HeLa nuclei treated with benzonase
Immuno-precipitation step Other - 2.5 mcg of SSRP1 were immobilized on 50 mcL of anti-mouse Dynabeads (Invitrogen) prior to IP
Username

Abcam user community

Verified customer

提交于 Feb 06 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"