Anti-SSRP1抗体[10D7] (ab26212)

概述

  • 产品名称Anti-SSRP1抗体[10D7]
    参阅全部 SSRP1 一抗
  • 描述
    小鼠单克隆抗体[10D7] to SSRP1
  • 特异性ab26212 recognises Structure specific recognition protein 1 (SSRP1).
  • 经测试应用适用于: ICC/IF, IP, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant SSRP1 fragment (Human)

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab26212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
IP 1/500. (see Abreview)
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
  • 序列相似性Belongs to the SSRP1 family.
    Contains 1 HMG box DNA-binding domain.
  • 结构域The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
  • 翻译后修饰Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
    Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
    Sumoylated.
  • 细胞定位Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Chromatin-specific transcription elongation factor 80 kDa subunit antibody
    • Cisplatin DNA SSRP antibody
    • Facilitates chromatin remodeling 80 kDa subunit antibody
    • Facilitates chromatin transcription complex 80 kDa subunit antibody
    • Facilitates chromatin transcription complex subunit SSRP1 antibody
    • FACT 80 kDa subunit antibody
    • FACT complex subunit SSRP1 antibody
    • FACT80 antibody
    • FACTp80 antibody
    • hSSRP1 antibody
    • Recombination signal sequence recognition protein 1 antibody
    • Recombination signal sequence recognition protein antibody
    • SSRP 1 antibody
    • SSRP1 antibody
    • SSRP1_HUMAN antibody
    • Structure specific recognition protein 1 antibody
    • Structure-specific recognition protein 1 antibody
    • T160 antibody
    see all

Anti-SSRP1 antibody [10D7] 图像

  • Anti-SSRP1 antibody [10D7] (ab26212) at 1 µg/ml + HeLa cell lysate

    Secondary
    Goat anti-mouse Ig conjugated to HRP.
    Developed using the ECL technique

    Predicted band size : 81 kDa
    Observed band size : 81 kDa
  • ICC/IF image of ab26212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLa cells stained with ab26212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-SSRP1 antibody [10D7] (ab26212)参考文献

ab26212 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Xenopus laevis Cell lysate - whole cell (Egg)
Specification Egg
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Feb 18 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 0.25 µg
Gel Running Conditions Non-reduced Denaturing (12% SDS-PAGE)
Sample Human Recombinant protein (Baculovirus expression)
Specification Baculovirus expression
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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提交于 Aug 30 2013

Thank you very much for your interest in ab26212. To our knowledge, ab26212 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab26212 now, test it in mouse and submit feedback to us in the form of an A...

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DISCOUNT CODE: XXXXXXXXX Expiration date: [06-March-2012] I am very pleased to hear you would like to accept our offer and test ab26212 in [Mouse]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer,...

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Thank you very much for your interest in ab26212. We do not currently have an image of ab26212 in mouse. Therefore, I can offer a discount off a future purchase if you buy ab26212 now, use it mouse and submit an image of your results to us in the f...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK293 cells)
Total protein in input 1e+006 cells
Specification HEK293 cells
Immuno-precipitation step Protein G
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提交于 Aug 18 2010

Application Western blot
Sample Mouse Cell lysate - whole cell (mES cells)
Loading amount 1 µg
Specification mES cells
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"