RabMAb

Anti-SSH3BP1 (phospho Y435)抗体[EP3954] (ab109395)

概述

  • 产品名称Anti-SSH3BP1 (phospho Y435)抗体[EP3954]
    参阅全部 SSH3BP1 一抗
  • 描述
    兔单克隆抗体[EP3954] to SSH3BP1 (phospho Y435)
  • 特异性ab109395 only detects SSH3BP1 phosphorylated at tyrosine 435.
  • 经测试应用适用于: WB, IP, ICC, Flow Cytmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding tyrosine 435 of Human SSH3BP1.

  • 阳性对照
    • Jurkat cell lysate treated with pervanadate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP 1/10 - 1/100.
ICC 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明Is unsuitable for IHC-P.
  • 靶标

    • 功能May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level.
    • 组织特异性Widely expressed, with highest expression in brain.
    • 疾病相关Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript.
    • 序列相似性Belongs to the ABI family.
      Contains 1 SH3 domain.
      Contains 1 t-SNARE coiled-coil homology domain.
    • 结构域The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.
    • 翻译后修饰In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl.
    • 细胞定位Cytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Abelson interactor 1 antibody
      • Abi-1 antibody
      • Abi1 antibody
      • ABI1_HUMAN antibody
      • Abl binding protein 4 antibody
      • Abl interactor 1 antibody
      • Abl interactor protein 1 antibody
      • Abl-binding protein 4 antibody
      • AblBP4 antibody
      • e3B1 antibody
      • Eps8 binding protein antibody
      • Eps8 SH3 domain binding protein antibody
      • Eps8 SH3 domain-binding protein antibody
      • Eps8-binding protein antibody
      • Hssh3bp1 antibody
      • Interactor protein AblBP4 antibody
      • NAP1 antibody
      • Nap1 binding protein antibody
      • Nap1-binding protein antibody
      • Nap1BP antibody
      • Spectrin SH3 domain binding protein 1 antibody
      • Spectrin SH3 domain-binding protein 1 antibody
      • SSH3BP antibody
      see all

    Anti-SSH3BP1 (phospho Y435) antibody [EP3954] 图像

    • All lanes : Anti-SSH3BP1 (phospho Y435) antibody [EP3954] (ab109395) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with pervanadate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 55 kDa

    Anti-SSH3BP1 (phospho Y435) antibody [EP3954] (ab109395)参考文献

    ab109395 has not yet been referenced specifically in any publications.

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