Anti-Abi-1抗体(ab65828)
Key features and details
- Rabbit polyclonal to Abi-1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Abi-1抗体
参阅全部 Abi-1 一抗 -
描述
兔多克隆抗体to Abi-1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Abi-1 aa 483-494 (C terminal).
Sequence:WYEGVCNRVTGL
Database link: Q8IZP0 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Lyophilized:0.2ml of distilled water will yield a concentration of 500µg/ml. -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.025% Sodium azide
Constituents: 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab65828于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml.
Heat Mediated Antigen Retrieval: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
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说明 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Heat Mediated Antigen Retrieval: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
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靶标
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功能
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. -
组织特异性
Widely expressed, with highest expression in brain. -
疾病相关
Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript. -
序列相似性
Belongs to the ABI family.
Contains 1 SH3 domain.
Contains 1 t-SNARE coiled-coil homology domain. -
结构域
The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A. -
翻译后修饰
In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. -
细胞定位
Cytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. - Information by UniProt
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数据库链接
- Entrez Gene: 10006 Human
- Entrez Gene: 11308 Mouse
- Entrez Gene: 79249 Rat
- Omim: 603050 Human
- SwissProt: Q8IZP0 Human
- SwissProt: Q8CBW3 Mouse
- SwissProt: Q9QZM5 Rat
- Unigene: 508148 Human
see all -
别名
- Abelson interactor 1 antibody
- Abi-1 antibody
- Abi1 antibody
see all
图片
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Abi 1 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65828 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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Abi 1 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65828 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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All lanes : Anti-Abi-1 antibody (ab65828) at 0.5 µg/ml
Lane 1 : rat brain tissue lysates
Lane 2 : mouse brain tissue lysates
Lane 3 : human THP-1 whole cell lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 55 kDa -
Immunohistochemical analysis of rat brain tissue labeling Abi-1 with ab65828 at 0.5 μg/mL.
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Anti-Abi-1 antibody (ab65828) at 2 µg/ml + Rat brain lysate
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab65828 被引用在 1 文献中.
- Gong LJ et al. Pinocembrin ameliorates intermittent hypoxia-induced neuroinflammation through BNIP3-dependent mitophagy in a murine model of sleep apnea. J Neuroinflammation 17:337 (2020). PubMed: 33176803