Anti-SSEA1抗体[MC-480] (ab16285)
Key features and details
- Mouse monoclonal [MC-480] to SSEA1
- Suitable for: IHC-FoFr, Flow Cyt, IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgM
概述
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产品名称
Anti-SSEA1抗体[MC-480] -
描述
小鼠单克隆抗体[MC-480] to SSEA1 -
宿主
Mouse -
特异性
We guarantee IHC-P in Human only
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经测试应用
适用于: IHC-FoFr, Flow Cyt, IHC-P, ICCmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human Hodgkin's Lymphoma Mouse embryonic carcinoma cell lines positive for SSEA1 include: F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25. Cell lines negative for SSEA1 include: PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147. ICC: Mouse embryonic stem cells. Flow Cyt: Mouse ES cells
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Tissue culture supernatant was cross flow concentrated. -
克隆
单克隆 -
克隆编号
MC-480 -
骨髓瘤
P3-x63-Ag8 -
同种型
IgM -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
- Goat F(ab')2 Anti-Mouse IgM mu chain (FITC) (ab5926)
- Goat F(ab')2 Anti-Mouse IgM mu chain (TRITC) (ab5928)
- Goat F(ab')2 Anti-Mouse IgM mu chain (Biotin) (ab5929)
- Goat F(ab')2 Anti-Mouse IgM mu chain (PE) (ab5932)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
- Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
- Goat Anti-Mouse IgM H&L (ab9167)
- Rabbit Anti-Mouse IgM mu chain (ab9175)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16285于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-FoFr |
Use at an assay dependent concentration.
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Flow Cyt | (2) |
Use at an assay dependent concentration.
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IHC-P |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
1/1000.
Fixation with 100% MeOH (5 min) or 4% PFA (10 min). |
说明 |
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IHC-FoFr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
1/1000. Fixation with 100% MeOH (5 min) or 4% PFA (10 min). |
靶标
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相关性
Stage-specific embryonic antigen-1 (SSEA-1) was described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter and Prof. Barbara Knowles (Solter D and Knowles B.B, 1978, PNAS 75:5565). The anti SSEA-1 monoclonal antibody detects a carbohydrate epitope, which is first present on blastomeres of the 8-cell mouse embryo. It is also present on the cell surface of mouse embryonic stem (ES) cells, mouse embryonal carcinoma (EC) cells and both mouse and human embryonic germ (EG) cells. SSEA1 expression decreases as mouse ES cells differentiate but increases during the differentiation of human ES and EC cells. This antigen is also found on the cell surface of human neutrophils and myeloid leukemias, where it’s expression pattern is likened to that of CD15. However, it is currently believed that the expression pattern of SSEA1 differs from CD15, especially on the early mouse embryo.
图片
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ab16285 staining SSEA1 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab16285 at a 1/1000 dilution and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150121, Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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IHC image of SSEA1 staining in Human Hodgkin’s Lymphoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was incubated with ab16285, 1/200 dilution, overnight at 4°C. A Goat anti-Mouse (IgM) HRP conjugated secondary antibody (ab97230 at 1/2000) was used to detect the primary and visualized using DAB as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).
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The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.
数据表及文件
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SDS download
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Datasheet download
文献 (60)
ab16285 被引用在 60 文献中.
- Yoshimoto Y et al. Tenogenic Induction From Induced Pluripotent Stem Cells Unveils the Trajectory Towards Tenocyte Differentiation. Front Cell Dev Biol 10:780038 (2022). PubMed: 35372337
- Ren Y et al. Knockout of integrin β1 in induced pluripotent stem cells accelerates skin-wound healing by promoting cell migration in extracellular matrix. Stem Cell Res Ther 13:389 (2022). PubMed: 35908001
- Ma B et al. Patient-specific and gene-corrected induced pluripotent stem cell-derived endothelial cells elucidate single-cell phenotype of pulmonary veno-occlusive disease. Stem Cell Reports 17:2674-2689 (2022). PubMed: 36400028
- Bayerl J et al. Principles of signaling pathway modulation for enhancing human naive pluripotency induction. Cell Stem Cell 28:1549-1565.e12 (2021). PubMed: 33915080
- Mao Y et al. Continuous expression of reprogramming factors induces and maintains mouse pluripotency without specific growth factors and signaling inhibitors. Cell Prolif 54:e13090 (2021). PubMed: 34197016