Anti-SSEA1抗体[MC-480] (ab16285)

概述

  • 产品名称Anti-SSEA1抗体[MC-480]
  • 描述
    小鼠单克隆抗体[MC-480] to SSEA1
  • 经测试应用适用于: IHC-FoFr, ICC/IF, IP, IHC-P, IHC-FrFl, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
    不与反应: Human
  • 免疫原

    F9 teratocarcinoma stem cells (X-irradiated).

  • 阳性对照
    • Mouse embryonic carcinoma cell lines positive for SSEA1 include: F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25. Cell lines negative for SSEA1 include: PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse normal brain.
  • 常规说明Isotype is mouse IgM, kappa light chain. From Solter and Knowles, 1978.

性能

应用

Our Abpromise guarantee covers the use of ab16285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration.
ICC/IF 1/100.
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

靶标

  • 功能May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.
  • 通路Protein modification; protein glycosylation.
  • 序列相似性Belongs to the glycosyltransferase 10 family.
  • 细胞定位Golgi apparatus, Golgi stack membrane. Membrane-bound form in trans cisternae of Golgi.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3)-fucosyltransferase 4 antibody
    • Alpha (1,3) fucosyltransferase antibody
    • Alpha (1,3) fucosyltransferase antibody antibody
    • Alpha-(1 antibody
    • CD15 antibody
    • EC=2.4.1. antibody
    • ELAM 1 ligand fucosyltransferase antibody
    • ELAM-1 ligand fucosyltransferase antibody
    • ELAM1 ligand fucosyltransferase antibody
    • Fuc TIV antibody
    • Fuc-TIV antibody
    • Fucosyltransferase 4 antibody
    • Fucosyltransferase IV antibody
    • FucT-IV antibody
    • FUCTIV antibody
    • Fut4 antibody
    • FUT4_HUMAN antibody
    • Galactoside 3 L fucosyltransferase antibody
    • Galactoside 3-L-fucosyltransferase antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA 1 antibody
    • SSEA-1 antibody
    • SSEA1 antibody
    • Stage specific embryonic antigen 1 antibody
    • Stage specific embryonic antigen 1 antibody antibody
    see all

Anti-SSEA1 antibody [MC-480] 图像

  • ICC/IF image of ab16285 stained mouse embryonic stem cells. The cells were 4% formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.
  • Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).

    See Abreview

  • IHC image of SSEA1 staining in mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16285, 10µg/ml, for 15 mins at room temperature. A Goat anti-Mouse biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SSEA1 antibody [MC-480] (ab16285)参考文献

This product has been referenced in:
  • Wang H  et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016). ICC/IF ; Pig . Read more (PubMed: 27264660) »
  • Hatzistergos KE  et al. cKit+ cardiac progenitors of neural crest origin. Proc Natl Acad Sci U S A 112:13051-6 (2015). IHC-P ; Mouse . Read more (PubMed: 26438843) »

See all 22 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Chicken Cell (Chicken ESC)
Permeabilization No
Specification Chicken ESC
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 06 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Zebrafish Cultured Cells (embryonic)
Specification embryonic
Fixative Formaldehyde
Permeabilization Yes - 0.5% tritone X 100
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Username

Miss. Salma Sana

Verified customer

提交于 Jan 16 2012

Thank you for your quick response.   ab119844 has been tested and characterized for ICC/IF so our Abpromise applies for this application.   If you need anything further or any help then please let me know.

Thank you for your enquiry and your interest in our products. I can confirm that CD15 and SSEA1 are alternative names and these antibodies detect the same protein. Depending when the original antibody was produced and the clone licensed, some ant...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (embryonic stem cell (D3))
Specification embryonic stem cell (D3)
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: enzyme-free
Fixation Formaldehyde
Permeabilization No
Gating Strategy live cells
Username

Abcam user community

Verified customer

提交于 Nov 24 2011

Thank you very much for your request. I have put the MSDS in the attachment of this email. The MSDS for ab19857 and ab16285 can also be directly downloaded from their datasheet. The link can be found scrolling down, underneath the "storage buffer". ...

Read More

DISCOUNT CODE: XXXXXXXXX Expiration date: 19-01-2012 I am very pleased to hear you would like to accept our offer and test ab16285 in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, plea...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Coronal Brain Sections)
Specification Coronal Brain Sections
Username

Abcam user community

Verified customer

提交于 May 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (mouse ES cell line D3)
Specification mouse ES cell line D3
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer (gibco) from adherent culture
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy FSC vs SSC on negative control sample
Username

Abcam user community

Verified customer

提交于 May 03 2011

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (F9 Cells)
Total protein in input 5000 µg
Specification F9 Cells
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

提交于 Oct 21 2009

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"