Anti-SSBP1抗体(ab116350)

概述

  • 产品名称Anti-SSBP1抗体
    参阅全部 SSBP1 一抗
  • 描述
    兔多克隆抗体to SSBP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 16-65 (HESETTTSLV LERSLNRVHL LGRVGQDPVL RQVEGKNPVT IFSLATNEMW) of Human SSBP1 (NP_003134).

  • 阳性对照
    • Human Fetal Kidney lysate.

性能

应用

Our Abpromise guarantee covers the use of ab116350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 17 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication.
  • 序列相似性Contains 1 SSB domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • mitochondrial antibody
    • Mt SSB antibody
    • Mt-SSB antibody
    • MtSSB antibody
    • PWP1 interacting protein 17 antibody
    • PWP1-interacting protein 17 antibody
    • SENSOR OF SINGLE-STRANDED DNA COMPLEX antibody
    • Single stranded DNA binding protein 1 antibody
    • Single stranded DNA binding protein antibody
    • Single stranded DNA binding protein mitochondrial antibody
    • Single-stranded DNA-binding protein antibody
    • SOSS B1 antibody
    • SOSS COMPLEX, SUBUNIT B1 antibody
    • SSBP 1 antibody
    • SSBP antibody
    • SSBP_HUMAN antibody
    • SSBP1 antibody
    see all

Anti-SSBP1 antibody 图像

  • All lanes : Anti-SSBP1 antibody (ab116350) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate- 18µg
    Lane 2 : HeLa whole cell lysate- 36µg
    Lane 3 : RPE-1 whole cell lysate- 18µg
    Lane 4 : RPE-1 whole cell lysate- 36µg

    Secondary
    LICOR, anti rabbit 800CW conjugated to IRDye® 800CW at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 17 kDa
    Additional bands at : 113 kDa (possible non-specific binding).

    Exposure time : 7 minutes

    This image is courtesy of an anonymous abreview.

    Blocking: 10% Milk

    See Abreview

  • Anti-SSBP1 antibody (ab116350) at 1 µg + Human Fetal Kidney lysate at 10 µg

    Predicted band size : 17 kDa
    Gel concentration: 10-20%.

Anti-SSBP1 antibody (ab116350)参考文献

ab116350 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 36 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - whole cell (HeLa, RPE-1)
Specification HeLa, RPE-1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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提交于 May 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"