概述

  • 产品名称Anti-SSB抗体
    参阅全部 SSB 一抗
  • 描述
    兔多克隆抗体to SSB
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: ISEDKTKIRR SPSKPLPEVT DEYKNDVKNR SVYIKGFPTD ATLDDIKEWL, corresponding to amino acids 82-131 of Human SSB

  • 阳性对照
    • Jurkat cell lysate and human liver and kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab50573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.
  • 序列相似性Contains 1 HTH La-type RNA-binding domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Phosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
    The N-terminus is blocked.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Autoantigen La antibody
    • La antibody
    • La autoantigen antibody
    • La autoantigen homolog antibody
    • La protein antibody
    • La ribonucleoprotein antibody
    • La ribonucleoprotein domain family member 3 antibody
    • LA_HUMAN antibody
    • LARP3 antibody
    • Lupus La antigen antibody
    • Lupus La protein antibody
    • Lupus La protein homolog antibody
    • MGC118101 antibody
    • MGC93380 antibody
    • mRNA for autoantigen antibody
    • OTTMUSP00000014043 antibody
    • RP23 273G23.1 antibody
    • Sjoegren syndrome type B antigen antibody
    • Sjogren syndrome antigen B (autoantigen La) antibody
    • Sjogren syndrome antigen B antibody
    • SS B antibody
    • SS-B antibody
    • SS-B/La protein antibody
    • SSB antibody
    see all

Anti-SSB antibody 图像

  • Anti-SSB antibody (ab50573) at 0.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • ab50573 at 4ug/ml dilution staining hepatocytes in human liver by Immunohistochemistry, Paraffin embedded tissue.
  • ab50573 at 4ug/ml dilution staining epithelial cells of renal tubule in human kidney by Immunohistochemistry, Paraffin embedded tissue.
  • ab50573 at 5µg/ml staining SSB in Human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab50573 at 5µg/ml staining SSB in Human prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • All lanes : Anti-SSB antibody (ab50573) at 2 µg

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 45 kDa

Anti-SSB antibody (ab50573)参考文献

ab50573 has not yet been referenced specifically in any publications.

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